中文摘要：

本研究以吖啶橙为分子探针pH=7.40,采用圆二色谱、紫外光谱等光谱法和粘度法等研究了酪氨酸-铒（Ⅲ）配合物与DNA作用机制.Er（Ⅲ）与Tyr的结合比nEr：nTyr=1∶3,Er（Ⅲ）（Tyr）3与DNA结合比nEr（Ⅲ）（Tyr）3：nDNA=2∶1.结合常数KΘ25℃=1.83×105 L.mol-1,KΘ37℃=4.31×105 L.mol-1.酪氨酸-铒配合物与DNA的主要作用方式为嵌插和沟渠作用,此反应受焓驱动.

英文摘要：

With the spectroscopy methods of CD spectra,UV spectra,fluorescence spectra and viscosity measurements in physiological pH environment（pH=7.40）,the binding ratio between Er（Ⅲ）,n（Tyr） and DNA in physiological pH environment（pH=7.40） were determined as nEr∶nTyr=1∶ 3,nEr（Ⅲ）（Tyr）3∶nDNA=2∶1.The binding constants of KΘ25 ℃=1.83×105 L·mol-1,KΘ37 ℃=4.31×105 L·mol-1 at different temperatures were confirmed by double reciprocal method.Thermodynamic function computation demonstrates that ΔrHΘm is the primary driving power of the interaction of Er（Ⅲ）（Tyr）3 and DNA.Combined with Scatchard method and viscosity method,the results suggest that the interaction mode between Er（Ⅲ）（Tyr）3 complex and herring sperm DNA are groove binding and intercalation binding.