中文摘要：

疣孢漆斑菌代谢产生的胆红素氧化酶在环境保护等领域有较大的应用潜力。为了提高胆红素氧化酶的产量,采用单因素试验和正交试验相结合的方法,研究培养基中C、N、金属离子及诱导剂对疣孢漆斑菌3.2190产酶的影响。结果表明,在产酶培养基中,葡萄糖和大豆蛋白胨为最适C源和N源,添加2mmol/L铜离子和0.1mmol/L的诱导剂均可以提高发酵液的酶活。正交试验得到的优化组合为：葡萄糖10g/L,大豆蛋白胨7.5g/L,CuSO41mmol/L,没食子酸0.1mmol/L,优化后发酵液酶活提高了3倍。初步纯化的胆红素氧化酶最适反应温度为40℃,最适pH值为7.5。在1000U/L的酶活下,只需50min就可以降解90%以上100mg/L的染料靛红,显示出胆红素氧化酶在染料废水处理中具有广阔的应用前景。

英文摘要：

Bilirubin oxidase （EC.1.3.3.5） produced by Myrothecium verrucaria shows a promising potential in the application of environmental protection.In order to increase the production of bilirubin oxidase by M.verrucaria,we investigated the effects of carbon,nitrogen,metal ions and inducers using the single-factor and orthogonal design methods.The results show that glucose and soya peptone are the best carbon and nitrogen sources,respectively.A higher enzyme activity was achieved with the addition of 2 mmol /L Cu2 ＋ and 0.1 mmol /L of inducers.The orthogonal design experiment showed that the optimal medium was glucose 10 g /L,soya peptone 7.5 g /L,CuSO4 1 mmol /L and gallic acid 0.1 mmol /L.The enzyme activity was enhanced three times under this optimal medium.The optimal temperature for the enzyme activity was 40℃ ,and optimal pH was 7.5.With an enzymatic activity of 1 000 U /L,the initial purified bilirubin oxidase could decolorize more than 90% of indigo carmine （100 mg /L） within only 50 min,suggesting a broad application prospect of bilirubin oxidase in dye effluent decolorization.