综述了自1993年首次报道细菌产漆酶以来相关细菌漆酶研究的主要成果,包括细菌漆酶的产生菌、酶活性测定、相关酶学特性,分子生物学及其工业应用的研究。细菌发酵的代时短、生物量大,细菌漆酶不需要糖基化、热稳定性好及碱性条件下具有较高的酶活。细菌漆酶作为新型的生物制剂在造纸工业等多个领域中具有重要的应用价值。
Laccases are found in fungi and plants. After bacterial lacease was firstly reported in 1993, more and more evidences have proved their distribution in prokaryotes by bioinformatics analysis and experimental data. In this paper, recent advances of research on bacterial lac- cases are reviewed from the following aspects : bacterial strains, the determination of the enzyme activity, enzymatic characteristics, molecu- lar biology and industrial application. The results show that bacteria can produce a high amount of laccase in a short time due to their fast growth speed. Compared with fungal laccases, there is no glycosylation modification in bacterial laccases, and they have higher thermal stability and enzyme activity in alkaline environments. Therefore, laccases produced by bacteria is of great significance in industrial applications such as environmental protection, food industry, paper bleaching, and so on.