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安佳欣胶囊对抑郁症模型大鼠基因功能类表达影响的初步研究

ISSN号：1002-0152
期刊名称：《中国神经精神疾病杂志》
时间：0
分类：R749.4[医药卫生—神经病学与精神病学;医药卫生—临床医学]
作者机构：[1]哈尔滨医科大学生物信息学系,哈尔滨150086, [2]成都康弘集团博士后工作站, [3]同济大学生命科学与技术学院
相关基金：国家自然科学基金资助项目（编号：30170515,30370388,30370798,30570424,30571034）;国家863计划资助项目（编号：2003AA222051;2002AA222052）;黑龙江科技攻关重点资助项目（编号：GB03C602-4）;哈尔滨市科技攻关资助项目（编号：2003AA3CS113）;黑龙江自然科学基金资助项目（编号：F0177）及哈医大211工程“十五”建设项目资助

作者：钟国才[1], 李强[2], 柯尊洪[2], 吕莹丽[1], 郝晓峰[2], 李崇前[2], 杨达[1], 王海敏[1], 王栋[3], 郭政[1]

关键词：安佳欣胶囊, 抑郁症, 基因表达谱, GENE, ONTOLOGY, 基因功能, ANJIAXIN capsule, depression, gene expression profile, Gene Ontology, gene function

中文摘要：

目的研究安佳欣胶囊对抑郁症模型大鼠基因功能类表达的影响。方法分析抑郁症大鼠模型组和安佳欣胶囊组各8例样本的基因表达谱数据，筛选差异表达基因。利用基因功能分类体系GeneOntology中的生物学过程子树和细胞组分子树，寻找和分析显著聚集差异表达基因的复合功能类，从分子水平和基因功能模块水平探索安佳欣胶囊对抑郁症模型大鼠基因表达谱的影响。结果筛选出330个差异表达基因，并进一步识别了8个差异表达基因功能模块，主要涉及糖代谢、蛋白转运、谷氨酰胺代谢、凋亡诱导和神经发生。通过文献证实进一步发现了差异功能模块中7个可能与抑郁症发病相关的基因（Pfkm、Gpx1、Stx1a、Ninj2、Pip、Ev1、Nrn1）在两组动物中差异表达。结论安佳欣胶囊可能增加抑郁症模型大鼠的单胺递质合成和释放，改善神经保护和神经发生功能，这些改变可能与安佳欣胶囊的抗抑郁作用有关。

英文摘要：

Objective To investigate the effect of ANJIAXIN capsule treatment gene expression profiles and gene functional modules of depression model rats. Methods Gene expression profiles were measured in 8 ANJIAXIN - treated depression model rats and 8 control model rats. Differentially expressed genes were identified and annotated to biological process and cellular component categories of Gene Ontology （GO） database. Then, GO categories enriched with significantly differentially expressed genes were identified to investigate the effect of ANJIAXIN capsule treatment at molecular level and gene functional module level. Results Three hundred and thirty differentially expressed genes were identified. Furthermore, eight gene functional modules with significantly gathered differentially expressed genes were recognized, which were mainly related to glycometabolism, protein transport, glutamine metabolism, induction of apoptosis and neurogenesis. Seven differentially expressed genes （Pfkm, Gpx1, Stx1a, Ninj2, Pip, Ev1, Nrn1） in the functional modules, which were potentially related to depression, were further identified through literature evidences. Conclusions The effect of ANJIAX- IN capsule treatment might be coordinately associated with enhancement of monoamine synthesis and release, and with improvement of neuroprotection and neurogenesis, which suggested the antidepression effect of ANJIAXIN capsule.

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