中文摘要：

以小球藻为原料,分离出色素蛋白复合体,并对其组成及抗氧化性质进行表征。用超声波破碎小球藻,收集类囊体膜后,加入两性离子表面活性剂CHAPS增溶,得到小球藻色素蛋白复合体。对色素蛋白复合体的组成进行分析,用HPLC法测定叶黄素含量,分光光度法测定叶绿素含量及BCA法测定蛋白质含量,结果表明：复合体中m叶黄素∶m叶绿素∶m蛋白=12.49∶7.18∶100。分离得到的小球藻色素蛋白复合体对DPPH自由基、超氧阴离子自由基的半数清除质量浓度分别为422μg/m L和294μg/m L;当其质量浓度为1 000μg/m L时,金属螯合率达30%;还原力随色素蛋白复合体质量浓度的上升而增强;复合体质量浓度600μg/m L时,可完全抑制脂质过氧化。

英文摘要：

The objective of this research are to isolate the pigment-protein complexes from chlorella and evaluate the antioxidant activities. Chlorella cells were broken by the ultrasonic wave in ice bath and the thylakoids were collected by ammonium sulfate precipitation. The pigment-protein complexes were extracted with phosphate buffer containing 1% of CHAPS. The lutein content of the complexes was analyzed by HPLC, the chlorophyll content was determined by spectrophotometry and BCA assay was employed to analyze the protein content. Results showed that the pigment-protein complexes of chlorella possessed a lutein ∶ Chl ∶ protein ratio of about 12.49 ∶ 7.18 ∶ 100（m/m/m）. The half maximal effective concentrations of the complexes against superoxide anion radical and DPPH radical were 294 μg/m L and 422 μg/m L respectively. The ferrous ion-chelating rate could reach 30% when the concentration of the complexes reach 1 000 μg/m L.The reducing power of the complexes was dose-dependent with concentration of 200-1 000 μg/m L. When the concentration of complexes reach 600 μg/m L, the lipid peroxidation could be completely inhibited.