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中文摘要：
铜离子对贝壳状革耳菌（Panusconchatus）胞外漆酶有明显的诱导作用，其最适诱导浓度为3mmol／L。在培养基中添加铜离子，发酵14d后漆酶活性提高11倍，达196IU／mL。发酵液经硫酸铵盐析，离子交换色谱和凝胶过滤色谱等分离纯化后，漆酶的比活力为912IU／mg，纯化倍数为6．77，酶活得率为74％；SDS—PAGE和Native—PAGE显示出单条蛋白条带，其分子量约是65kDa；以ABTS为底物时Km为0．0057mmol／L；反应最适温度和pH分别为60℃和2．5；漆酶在4℃和pH8．0时具有较高的稳定性；K＋，Na＋，Cu＋，Mg2＋等金属离子和乙醇，乙腈对酶活影响较小；而SDS、半胱氨酸和NaN，等对酶活影响较大，几乎完全抑制漆酶活性。
英文摘要：
Laccase production by Panus conchatus reached the highest enzyme activity 196 IU/mL at 14th day under induction of 3 mmol/L Cu2＋ . The enzyme was purified by ammonium sulfate precipitation, anion-exchange chroma- tography and size-exclusion chromatography. Purification of about 6.77-fold was achieved with an over yield of 74 % and a specific activity of 912 IU/mg. A single laccase band by Native-PAGE was found with a molecular mass of 65 kDa by SDS - PAGE. The Michaelis constant of the enzyme for ABTS was 0. 0057 mmol/L, the optimal temper- ature and pH for the enzyme activity are 60 ℃ and 2.5. The laccase was stable under 4 ℃ and pH 8.0, and resist- ant to metal ions such as K＋ , Na＋ , Cu2＋ , Mg2＋ and other chemicals such as ethanol and acetonitrile, but is com- pletely inhibited by SDS, cysteine and NaN3.
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