欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的探讨细胞周期对于大鼠局灶性脑缺血后神经元的影响。方法采用MCAO方法制作大鼠局灶性脑缺血模型，应用免疫荧光技术观察缺血后1d、3d、7d、14d大鼠缺血侧病灶周围神经元中磷酸化细胞周期蛋白CDK2、CDC2及磷酸化Rb的表达。结果与正常对照组相比，缺血后1d、3d组磷酸化CDK2和磷酸化Rb的表达量明显增加（P〈0．05）。缺血后7d、14d组磷酸化CDK2和磷酸化Rb的表达量无增加。磷酸化CDC2在正常组及缺血组均无明显表达。结论大鼠局灶性脑缺血后早期部分神经元再次进入细胞周期，提示细胞周期调控参与了大鼠局灶性脑缺血后神经元的凋亡。
英文摘要：
Objective To observe the expression of phosphorylated cell cycle-related proteins and the activity of the retinoblastoma tumor suppressor protein （Rb） after focal cerebral ischemia in ratsi Methods We used immunofluorescence staining for anti-phospho-CDK2, anti-phospho-CDC2, anti-phospho-Rb and anti-NeuN antibodies to determine their expression in the cortex of Wistar rats 1 d, 3d, 7d and 14d after focal cerebral ischemia. Results The expression of phospho-CDK2 and phospho-Rb increased in the neurons near the infarction in the ld and 3d groups when compared with that of the control group. In the 7d and 14d groups, there was no significant change of expression. In all groups, there were no expression of phospho-CDC2. Conclusion The expression of phospho- CDK2 and phospho-Rb suggests that differentiated neurons can re-enter the cell cycle, and more neurons enter the cell cycle in the early stage after ischemia. Re-entering of differential neurons into the cell cycle might participate in apoptosis.
同期刊论文项目
同项目期刊论文
Erythropoietin prevents PC12 cells from 1-methyl-4-phenylpyridinium ion-induced apoptosis via the Ak
期刊信息
位置：