中文摘要：

为探讨星形胶质细胞（Ast）在癫痫发病机制中的作用，本研究通过体外分离纯化培养分别获取大鼠大脑皮质Ast及神经元，用睫状神经营养因子（CNTF）激活Ast，用其培养液，即星形胶质细胞条件培养液（ACM）孵育神经元4、8、12h后，采用Westernblot法及图像分析技术观察ACM对神经元内表达钙调蛋白依赖性激酶Ⅱ（CaMKⅡ）、诱导型一氧化氮合酶（iNOS）及腺苷酸环化酶（AC）的影响。结果表明，ACM作用神经元4h即可引起CaMKⅡ、iNOS及AC的表达增多并持续至12h（P〈0．05），iNOS在8h最为明显，AC的表达在12h最为明显。上述结果提示ACM中含有致痫因子，NOS-NO—cGMP，Ca^2＋／CaM CaMKⅡ及AC—CaMP-PKA三条信号通路可能参与其致痫机制中的信号转导。

英文摘要：

To explore the roles of astrocytes in the epileptogenesis, firstly, we gained astmcytes and neurons respectively from cerebral cortex of rats by isolation, purification, and culture. Then, astrocytes activated by ciliary neurotrophic factor （CNTF）, the medium, i. e. , astrocytic conditioned medium （ACM） was collected to treat neurons for 4, 8 and 12 h, respectively. After that, Western blot was performed to observe the expressions of calmodulin dependent protein kinase Ⅱ （ CaMK Ⅱ ） , induced nitricoxide synthase （ iNOS ） and adenylate cyclase （AC） in neurons. The results showed that the expressions of CaMK Ⅱ, iNOS and AC were increased significantly from 4 h to 12 h following treatment of ACM （P 〈 0.05 ）, and iN OS peaked at 8 h, while AC at 12 h. These results suggest that there might be some epileptogenic factors in the ACM and NOS-NO-cGMP, Ca^2 ＋/CaM-CaMK Ⅱ and AC-cAMP-PKA related signal transduction pathways might take part in the signal transduction of epileptogenesis.