中文摘要：

目的：建立同时测定葛根芩连汤中葛根素、大豆苷、甘草苷、黄连碱、药根碱、黄芩苷、小檗碱、巴马汀、汉黄芩苷、黄芩素、汉黄芩素和甘草酸铵12个有效成分含量的HPLC-DAD测定方法。方法：采用高效液相色谱法,色谱柱MerckChromolith,RP-18e（4.6 mm×100 mm）,流动相为乙腈-0.05%磷酸（含0.01 mol.L^-1磷酸二氢钾）梯度洗脱;流速2 mL.min^-1,柱温35℃,检测波长250,275,345 nm。结果：12个成分的方法学考察均合格,阴性无干扰。结论：本方法简便、快速、准确可靠,专属性强,可用于葛根芩连汤的质量控制。

英文摘要：

Objective：To establish a HPLC-DAD method for simultaneous determination of puerarin,daidzin,liquiritin,coptisine hydrochloride,jatrorrhizine hydrochloride,baicalin,berberine hydrochloride,palmatine hydrochloride,wogonoside,baicalein,wogonin and ammonium glycyrrhizinate in Gegenqinlian decoction.Method：A Merck Chromolith RP-18e（4.6 mm × 100 mm） was used;the mobile phase was acetonitrile-0.05% phosphoric acid（contain 0.01 mol.L-1 potassium dihydrogen phosphate） in gradient elution.The detection wavelength was set at 250,275,345 nm,the flow rate was 2.0 mL.min-1 and the column temperature was maintained at 35 ℃.Result：The accuracy,precision,sensitivity,specificity and linearity of this method met the requirements.The content of the twelve active components was determined simultaneously.Conclusion：The method is rapid,simple and accurate,it has a good specificity and it can be suitable for the determination of twelve active components in Gegenqinlian decoction simultaneously.