为明确海洋甲藻——网状原角藻(Protoceratium reticulatum)对海洋微藻的化感作用,揭示其化感物质作为植物源抑藻剂的开发潜能,本文探讨了网状原角藻藻细胞和无细胞滤液对几种海洋微藻——东海原甲藻(Prorocentrum donghaiense)、拟旋链角毛藻(Chaetoceros pseudocurvisetus)、米氏凯伦藻(Karenia mikimotoi)和赤潮异弯藻(Heterosigma akashiwo)生长的影响,并进一步选取东海原甲藻和赤潮异弯藻作为受体藻种,以塔玛亚历山大藻(Alexandrium tamarense)作为阳性对照,比较了2种甲藻的藻细胞培养液和无细胞滤液对东海原甲藻、赤潮异弯藻生长的影响差异。通过比较发现,网状原角藻的无细胞滤液比塔玛亚历山大藻能更明显的抑制东海原甲藻和赤潮异弯藻的生长,尤其是对东海原甲藻,基本阻断其生长。
To investigate the allelopathic activity of Protoceratium reticulatum,experiments were designed to study the allelopathic effect of cells and cell free used medium of Protoceratium reticulatum on the growth of other marine microalgae,such as Prorocentrum donghaiense,Chaetoceros pseudocurvisetus,Karenia mikimotoi and Heterosigma akashiwo.In addition,Alexandrium tamarense was chosen as apositive control and P.donghaiense,H.akashiwo were chosen as the target species in order to estimate the strength of allelopathic activity of P.reticulatum.The results showed that the growth of P.donghaiense and H.akashiwo was affected obviously by donor species each when cocultured.The cell free used medium of P.reticulatum had a stronger influence on the growth of the two target species,especially on that of P.donghaiense as its growth was almost completely blocked.