中文摘要：

目的探索哇巴因（Ouabain）对人胶质瘤U87-MG细胞增殖和缺氧诱导因子-1α（HIF-1α）表达的影响。方法Ouabain（0.05、0.5、2.5、25μmol/L）作用于U87-MG细胞24 h,MTT法检测吸光度值和细胞活力,Western Blot检测HIF-1α表达量。结果不同浓度Ouabain干预24 h后U87-MG细胞吸光度值（OD490）与Control组相比,均明显降低（P均〈0.01）,细胞活力显著下降（P〈0.01）;与Control组比较,0.05μmol/L Ouabain组HIF-1α蛋白表达量上升（1.27±0.05vs.1.00±0.05,P〈0.05）,当Ouabain浓度升高至2.5、25μmol/L,HIF-1α表达显著降低（0.28±0.04,0.20±0.03 vs.1.00±0.05,P均〈0.01）。结论较高浓度Ouabain可通过抑制HIF-1α表达,促进人胶质瘤U87-MG细胞凋亡。

英文摘要：

Objective To explore the effects of Ouabain on the growth of human glioma U87-MG cells and the expression of hypoxia inducible factor-1α（ HIF-1α）. Methods U87-MG cells were treated with different concentrations of Ouabain（ 0. 05,0. 5,2. 5,25 μmol/L） for 24 h. MTT assay and Western Blot were used to detect cell viability and survival rate and the protein expression of HIF-1α,respectively. Results Compared with the control group,the optical density value（ OD490） and cell viability of U87-MG cells which were treated with different concentrations of Ouabain were significantly decreased（ all P 〈 0. 01）. Compared with the control group,0. 05 μmol/L Ouabain up-regulated the protein expression of HIF-1α（ 1. 27 ± 0. 05 vs. 1. 00 ± 0. 05,P〈 0. 05）,while 2. 5 and 25 μmol/L Ouabain significantly decreased the protein level of HIF-1α（ 0. 28 ±0. 04,0. 20 ± 0. 03 vs. 1. 00 ± 0. 05,both P 〈 0. 01）. Conclusion Ouabain with high dose could promote the apoptosis of human glioma U87-MG cells by down-regulating HIF-1α.