中文摘要：

目的 探讨哇巴因（ouabain）对人胶质瘤U87-MG细胞死亡的影响及具体机制。方法 将培养的U87-MG细胞随机分为正常对照（Control）组和ouabain组,分别加入常规培养基和不同浓度ouabain（0.05、0.5、2.5、25μmol/L）干预24 h,光学显微镜观察ouabain干预后24 h细胞的形态改变,MTT实验检测细胞活力,免疫印迹法（Western blot）检测Akt、p-Akt、m TOR和p-m TOR的表达变化。结果 与Control组比较,ouabain显著降低U87-MG细胞活力（均P〈0.01）,且呈现浓度依赖性;高浓度ouabain（2.5、25μmol/L）与Control组相比能够降低U87-MG细胞中p-Akt、m TOR和p-m TOR的表达（均P〈0.01）。结论 高浓度ouabain可能通过抑制胞内Akt/m TOR信号通路,降低肿瘤细胞活力并最终引起细胞死亡。

英文摘要：

Objective To explore the effect of ouabain on the human glioma U87-MG cells death and the underlying mechanism. Methods U87-MG cells were randomly divided into control group and ouabain group, which were treated with conventional medium and different concentrations of ouabain （0.05, 0.5, 2.5 and 25 μmol/L） for 24 h, respectively. The morphologic changes induced by ouabain treatment were observed with the light microscope. Cell viability was assessed with MTT assay and Western blot was used to detect the protein expression of Akt, p-Akt, mTOR and p-roTOR. Results Compared to control group, the viability of U87-MG cells treated with ouabain was significantly reduced （all P 〈 0.01） in a dose-dependent manner. Besides, Western blot indicated that higher concentrations of ouabain （2.5 and 25 μmol/L） downregulated the expression of p-Akt, mTOR and p-mTOR when compared to control group （all P 〈 0.01）. Conclusion Higher concentrations of ouabain depressed U87-MG cells viability and ultimately induced cell death via suppression of Akt/mTOR signaling pathway.