实验以三氯甲烷-甲醇-水(体积比30∶8∶1)为展开剂(二次展开),采用薄层层析法分离黄芪中的黄芪甲苷和三七中的人参皂苷,所得TLC谱图清晰,黄芪甲苷、人参皂苷分离度好(除人参皂苷Re与三七皂苷R1斑点重叠外,其余各斑点之间分离度均大于1.5).
Astragaloside PC in Radix Astragali and Ginsenoside in Radixet Rhizoma Notoginseng were analyzed by TLC with the silica gel plate in chloroform-methanol-water (30 : 8 : 1) after twice development. The TLC spots were highly specific,clear and concentrated,without interference by the presence of other chemical components.