中文摘要：

以大米与小麦粒作为主要材料,设计4组培养基栽培双梭孢虫草（Cordyceps bifusispora）,比较子实体干重。采用高效液相色谱法测定子实体中虫草菌素和腺苷含量,采用3,5-二硝基水杨酸（DNS）法测定多糖含量。结果表明：在室内自然条件下,依据子实体干重,双梭孢虫草较适宜的栽培培养基为小麦粒加营养液1（20 g葡萄糖,5 g蛋白胨,3 g KH2PO4,1.5 g MgSO4,5 mg VB1）；子实体的虫草菌素含量（103.1±15.7-122.1±18.6μg/g）各实验组间无显著性差异；依据子实体腺苷含量,适宜栽培培养基为小麦粒加营养液2（10 g葡萄糖,10 g酵母膏,1 g CaCl2,0.5 g MgSO4,0.5 g ZnSO4,0.1 g VB1）,为1337.5±75.2μg/g；依据子实体多糖含量,适宜栽培培养基为大米加营养液2,为251.1±0.7 mg/g。

英文摘要：

Fruit body yields, together with cordycepin, adenosine and polysaccharide contents, were determined following cultivation of Cordyceps bifusispora on four different media containing either rice or wheat as the major component.Highest fruit body yields were recorded following cultivation on a medium consisting of 30 g wheat and 40 mL of a nutrient solution containing （per litre）20 g glucose,5 g peptone,3 g KH2 PO4 ,1.5 g MgSO4 and 5 mg vitamin B1 .No significant differences in the cordycepin levels （103.1 ± 15.7-122.1 ± 18.6 μg/g）were observed in fruit bodies cultivated on all four media,whereas highest adenosine levels （1337.5±75.2μg/g）were recorded following cultivation on a medium consisting of 30 g wheat and 40 mL of a nutrient solution containing （per litre）10 g glucose,10 g yeast extract,1 g CaCl2 ,0.5 g MgSO4,0.5 g ZnSO4and 0.1 g vitamin B1.Highest polysaccharide levels （251.1±0.7 mg/g）were recorded in fruit bodies grown on a medium consisting of 30 g rice and 40 mL of a nutrient solution containing （per litre）10 g glucose,10 g yeast extract,1 g CaCl2 ,0.5 g MgSO4 ,0.5 g ZnSO4 and 0.1 g vitamin B1 .