中文摘要：

目的研究阿托伐他汀片预处理对脑缺血再灌注大鼠脑组织髓鞘碱性蛋白（MBP）、神经胶质纤维酸性蛋白（GFAP）和神经元特异性烯醇化酶（NSE）水平的影响。方法 30只雄性SD大鼠随机分为实验组、模型组、假手术组,每组10只。实验组术前5 d以阿托伐他汀混悬液5mg·kg^-1·d^-1灌胃,每天1次;模型组、假手术组于术前5天以等量0.9%Na Cl溶液进行灌胃,每天1次。实验组、模型组建立右侧大脑中动脉闭塞缺血再灌注模型,假手术组仅分离右侧颈总动脉后清创缝合。各组分别于缺血再灌注24 h后取脑并用免疫组化方法检测梗死灶周围MBP、GFAP及NSE水平。结果假手术组脑组织NSE为0.18±0.02,GFAP为0.11±0.00,MBP为0.19±0.02;模型组NSE为0.11±0.03,GFAP为0.14±0.04,MBP为0.11±0.02,与假手术组相比,差异均有统计学意义（P〈0.05）;实验组NSE为0.14±0.02,MBP为0.14±0.02,与模型组相比,差异有统计学意义（P〈0.05）;实验组GFAP与模型组相比,差异无统计学意义（P〉0.05）。结论阿托伐他汀预处理有利于减轻脑缺血再灌注损伤,其作用可能与阿托他汀对大鼠神经元、少突胶质细胞的保护有关。

英文摘要：

Objective To investigate the effect of atorvastatin preconditioning on cerebral myelin basic protein（ MBP）,glial fibrillary acidic protein（ GFAP） and neurospecific enolase（ NSE） in rat model of cerebral ischemia reperfusion. Methods A total of 30 male SD rats were randomly assigned to test group,model group and sham group. The rats of test group received atorvastatin 5mg·kg^-1·d^-1 gastric gavage for 5 consecutive days before modling while the other two groups received the same volume of 0. 9% Na Cl. Right middle cerebral artery occlusion（ MCAO） ischemia- reperfusion model was established in both model group and test group,while sham group was only subjected to right middle cerebral artery separation and suture. The expressions of cerebral NSE,MBP and GFAP were measured with immunohistochemistry after 24 h reperfusion. Results The expressions of NSE,MBP and GFAP were0. 11 ± 0. 03,0. 11 ± 0. 02,0. 14 ± 0. 04 in model group,had significant differences with those in sham group, which were 0. 18 ± 0. 02,0. 11 ± 0. 00,0. 19 ± 0. 02（ P〈0. 05）. The expressions of NSEand MBP in test group were 0. 14 ± 0. 02,0. 14 ± 0. 02,had significant differences with those of model group（ P〈0. 05）. The expression of GFAP in test group had no statistical significance with model group（ P〉0. 05）.Conclusion Atorvastatin preconditioning can alleviate cerebral ischemia reperfusion injury in rats with MCAO,probably through protecting oligodendrocytes and neurons.