中文摘要：

目的观察慢性应激对大鼠外显行为学变化及特异性蛋白——神经颗粒素（Ng）表达的影响，探讨三七皂苷Rg1对应激后认知功能损害的防治效果。方法成年雄性SD大鼠36只，按随机数字表法分为对照组、模型组（cus组）和治疗组（cus-G组），每组12只。采用慢性不可预见性应激方法建立慢性应激动物模型，采用体质量测量、糖水实验检测行为学变化，Morris水迷宫实验进行学习记忆力测试．Westernblotting法俭测皮层、海马Ng含量，免疫组化染色检测Ng表达，HE染色观察动物模型目标区域的形态学变化。结果CUS组大鼠体质黾明显减少，糖水消耗量明晟降低，与对照组、CUS—G组比较差异均有统计学意义（P〈0．05）。水迷宫实验显示应激动物逃避潜伏期明显延长，而CUS—G组大鼠逃避潜伏期呈下降趋势，与对照组、CUS组比较差异均有统计学意义（P〈0．05）。与较对照组大鼠比较．CUS组大鼠皮层、海马Ng含量明显降低，免疫组化染色实验中吸光度值明显下降；CUS—G组大鼠则较CUS组明显上升，差异均有统计学意义（P〈0．05）。结论100mg／kg剂量的三七皂苷Rg1能显著升高慢性应激模型大鼠Ng在皮层、海马的表达，对应激所致外届行为有积极的调节作用。

英文摘要：

Objective To investigate the effect of chronic stress on rat behavior changes, and specific protein neurogranin （Ng） level changes to explore the control efficiency ofginsenoside Rgl in cognitive impairment. Methods Thirty-six adult male SD rats were randomly divided into control group, model group （chronic unpredictable stress animal models, CUS）, and CUS-G treatment group. The chronic stress models in the later two groups were established by CUS; rats in the CUS-G treatment group were given 1100 mg/kg ginsenoside Rgl; Behavior changes of rats were detected by sugar consumption test and body weight measuring. Morris water maze test was used to study the learning and memory abilities. The Ng content in the cortex and hippocampus was detected by Western blotting. The Ng expression in the cortex and hippocampus was measured by immumohistochemical staining. Morphological changes in the target areas of animal models were detected by HE staining. Results As compared with the control group and CUS-G treatment group, the CUS model group had significantly decreased sugar consumption and weight （P〈0.05）. The water maze test showed that learning and memory abilities in rats decreased significantly after chronic stress, and the escape latency in the CUS-Gtreatment group was reduced, which showed significant difference as compared with that in the control group and CUS model group （P〈0.05）. As compared with the control group, the CUS model group had significantly decreased Ng content in the cerebral cortex and hippocampus and average absorbance values ofNg （P〈0.05）, while the CUS-G treatment group had significantly increased Ng content in the cerebral cortex and hippocampus and average absorbance values of Ng as compared with the CUS model group （P〈0.05）. Conclusions Ginsensode Rgl （100 mg/kg） can increase the level of Ng in the cerebral cortex and hippocampus to restore the damage of cognitive ability.