中文摘要：

目的：探讨甲强龙对实验性变态反应性脑脊髓炎（ EAE）小鼠的肿瘤坏死因子α（ TNF－α）和白介素17（IL－17）的影响。方法将30只雌性C57BL／6J小鼠随机分为甲强龙组、EAE组和正常组，每组10只。 EAE模型通过以髓鞘少突胶质细胞糖蛋白多肽35－55（ MOG35－55）为抗原诱导。采用蛋白质印迹法（ Western Blot ）检测小鼠大脑的TNF－α和 IL－17表达水平。结果甲强龙组的临床症状较EAE组改善（ P＜0．05），大脑的TNF－α和IL－17表达水平明显低于EAE组（ P＜0．05）。结论甲强龙能改善EAE小鼠的症状，其作用机制之一是抑制促炎性因子TNF－α和IL－17的表达。

英文摘要：

Objective To explore the effects of methylprednisolone on the levels of TNF-αand IL-17 in the central nervous system of mice with experimental autoimmune encephalomyelitis （ EAE ） .Methods 30 female C57BL/6J mice were randomly divided into 3 groups： methylprednisolone-treated group, EAE group and normal group.The EAE model was induced by immunizing mice with myelin oligodendrocyte glycoprotein peptides （ MOG35-55）.The brain tissues were collected and their expressions of TNF-αand IL-17 were detected using Western Blot . Results The neurological deficits of the methylprednisolone-treated mice were lighter than those of the EAE mice （ P〈0.05 ） , while the methylprednisolone-treated mice displayed the lower expressions of TNF-αand IL-17 than the EAE mice did（P〈0.05）.Conclusion Methylprednisolone can protect against EAE in mice by the inhibition of TNF-αand IL-17 .