为探索菌根-土壤-植物共生体系原理,以马尾松为供试植物,研究褐环乳牛肝菌Sl12、Sl13对苗木根际和非根际土壤pH值、酶活性和可培养微生物数量的影响。结果表明:(1)褐环乳牛肝菌接种处理会使苗木根际土壤的pH值降低0.13。(2)褐环乳牛肝菌在不同程度上增加根际土壤过氧化氢酶、蔗糖酶、脲酶、磷酸酶的活性,与CK相比,Sl13菌株根际土壤酶活性分别增加47.14%、8.86%、5.91%、22.38%。(3)接种褐环乳牛肝菌能显著增加根际土壤细菌、放线菌、真菌的数量。其中,Sl12菌株能显著增加土壤中可培养真菌数量,提升效果优于Sl13。与CK相比,接种组Sl12菌株根际真菌数量增加63.75%,Sl13菌株增加39.06%。研究表明褐环乳牛肝菌通过改变马尾松苗木的根际土壤微环境,从而促进植物生长发育,提高植物抗逆性。
To investigate the principle of the mycorrhizal soil plant symbiosis system, the Pinus massoniana seedings were used as the test plant.In order to study the influence of Suillus luteus, Sl12 and Sl13 inoculation on the soil pH,the soil enzyme activity and the microbial populations in the rhizosphere and non-rhizosphere. The results showed that ectomycorrhiza could decrease the soil pH value 0.13 and increase the activity of soil catalase, sucrase, urease and phosphatase in rhizosphere in different degree by inoculation with Suillus luteus. Compared with the control group, Sl13 significantly increased the soil enzyme activity, up to 47.14%、8.86%、5.91%、22.38%.The number of bacteria, actinomyces, azotobacter were significantly increased in rhizosphere by inoculated the Suillus luteus. Among them. Sl12 could obviously expand the number of bacteria, and Sl12 was better than Sl12. compared with the control group,the number of bacteria added to 63.75% and 39.06% by inoculated the Suillusluteu Sl12、Sl13. The results indicated that Suillusluteus could improve the soil environment of Pinus massoniana seedings、promote the plant growth and development, improve resistance of plants.