中文摘要：

目的探讨小续命汤有效成分对慢性脑缺血大鼠氧化应激损伤及神经细胞凋亡的影响。方法采用永久性结扎双侧颈总动脉法建立慢性脑缺血大鼠模型，将造模成功的大鼠按随机数字表法分为模型组、银杏叶提取物组和小续命汤有效成分低、中、高剂量组；另设假手术组作为对照。采用分光光度法检测各组大鼠脑组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量的变化，TUNEL法检测神经细胞凋亡情况，Westemblotting检测细胞凋亡相关蛋白caspase-3、Bcl-2、Bax的表达变化。结果与模型组相比，低剂量和中剂量小续命汤有效成分可显著抑制慢性脑缺血大鼠长期缺血缺氧导致的氧化应激反应，表现为降低脂质过氧化水平及增强脑组织抗氧化酶GSH-Px活性，差异有统计学意义（P＜0．05），但对SOD和CAT活性无显著影响。与模型组相比，中剂量小续命汤有效成分可显著减少慢性脑缺血大鼠神经细胞的凋亡率，同时低剂量和中剂量小续命汤有效成分还可显著升高脑组织Bcl-2／Bax比值，抑制下游Caspase凋亡级联反应，差异均有统计学意SL（P〈0．05）。结论小续命汤有效成分可显著抑制慢性脑缺血导致的氧化应激损伤，减少神经细胞凋亡。

英文摘要：

Objective To investigate the effect of active components of Xiaoxuming decoction（XXM） on oxidative stress and neuronal apoptosis in rats with cttromc cereorat lscnemaa. Bilateral common carotid arteries were permanently occluded in rats to establish the chronic cerebral ischemia models. The rats with chronic cerebral ischemia were randomly divided into five groups： vehicle group, Ginkgo biloba extract （EGB） group, and low-, medium- and high- dose active components of XXM groups. The sham-operated rats were treated with saline. The activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, catalase and the malondialdehyde （MDA） content were detected with spectrophotometry. TUNEL assay was used to measure the neuronal apoptosis. Western blotting was used to observe the expressions of neuronal apoptosis related proteins such as Caspase-3, Bcl-2 and Ban. Results The low-dose and medium-dose active components of XXM could significantly inhibit oxidative stress, decrease lipid peroxide, and attenuate the decreased activities of GSH-Px in 2VO rats （P〈0.05）. The medium-dose active components of XXM markedly prevented neurons from apoptosis （P〈0.05）. In addition, the low-dose and medium-dose active components of XXM increased Bcl-2/Bax ratio and decreased Caspase-3 mediated downstream cascade reaction （P〈0.05）.Conclusion The active components of XXM can inhibit oxidative stress and decrease neuronal apoptosis in rats with chronic cerebral ischemia.