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中文摘要：
匹诺塞林是一种广泛存在于蜂胶和植物中的黄酮类天然化合物,具有抗菌、抗炎、抗氧化、神经保护等多种药理作用。本实验利用大鼠全脑缺血模型（4-vessel occlusion,4-VO）,评价了匹诺塞林在体内对4-VO大鼠脑线粒体功能的保护作用。结果表明,匹诺塞林能显著提高4-VO大鼠脑线粒体的ADP/O比值、V3以及RCI、OPR等参数,保护线粒体结构完整性;体外实验表明,匹诺塞林能显著提高分离的脑线粒体的ADP/O、V3、RCI、OPR,降低V4,保护线粒体结构完整性;同时,匹诺塞林在体外条件下促进脑线粒体ATP的生成;在细胞水平,匹诺塞林能促进SH-SY5Y细胞的ATP生成。本研究发现,匹诺塞林通过保护线粒体结构完整性,降低NADH呼吸链的电子漏流,提高氧化磷酸化效率,促进线粒体呼吸功能,提升线粒体ATP合成能力等起到脑缺血保护作用。
英文摘要：
There are growing evidences that pinocembrin has better neuroprotective effect.In the present study,the effect of pinocembrin on mitochondrial respiratory function was evaluated in global brain ischemia/ reperfusion（4-vessel occlusion,4-VO） rats.The results showed that pinocembrin improved the respiratory activity of 4-VO brain mitochondria,through increasing ADP/O,state 3 respiration state（V3）,respiration control rate index（RCI） and oxidative phosphorylation rate（OPR）.And then,the effect of pinocembrin on brain mitochondria was verified in vitro.The results showed that pinocembrin increased ADP/O,state 3 respiration state,respiration control rate index,oxidative phosphorylation rate in NADH/FADH2 dependent respiratory chain and decreased state 4 respiration state（V4） in NADH dependent respiratory chain.Pinocembrin improved ATP content in brain mitochondria in vitro and in SH-SY5Y cells.
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