中文摘要：

本研究采用扩增条件优化的PCR扩增技术,以MDCC-MSB1细胞基因组DNA为模板扩增出鸡端粒酶RNA（chicken telomerase RNA,chTR）全长基因,克隆到pMD18-T载体中,经酶切鉴定和PCR鉴定后测定序列。序列分析表明所克隆的鸡端粒酶RNA基因全长465bp,其中模板区的11个核苷（5＇-CUAACC-CUAAU-3＇）合成端粒亚单位（TTAGGG）n。chTR基因的克隆为进一步研究chTR在马立克氏病发病过程中的作用以及马立克氏病的发病机制提供可能的序列基础。

英文摘要：

The chicken telomerase RNA intact gene was obtained by optimizing PCR reaction system using MDCC-MSB 1 genomic DNA as a template, and cloned it into plasmid vector pMD18-T. The product of the PCR was analyzed by sequencing after identification by PCR and restriction enzyme digestion. The sequence analysis indicated that the full length of chicken telomerase RNA intact gene was 465 bp, in which there were 11 nucleotides, 5＇-CUAACCCUAAU-3＇, encompassed template region of chicken telomerase RNA and composed the subunits of telomerase （TTAGGG）,. The cloning of chicken telomerase RNA may offer some possible sequences for finding out the roles of chicken telomerase RNA in the process of Marek＇s disease virus and researching on the pathogenesis of Marek＇s s disease virus.