为了探讨饲料中糊精水平对乌克兰鳞鲤生长和糖代谢的影响,试验配制蛋白质水平为30%,糊精水平分别为15%和25%的2种试验饲料,以平均体重为3.92 g的乌克兰鳞鲤幼鱼为试验对象,将300尾鱼随机分为2组,每组设3个重复,每个重复50尾鱼。饲养8周后,检测乌克兰鳞鲤生长指标、生化指标和糖代谢酶活性;禁食48 h后再投喂,测定投喂后0、3、6、12、24、48 h肝胰脏和肠道葡萄糖激酶(GK)和葡萄-6-磷酸酶(G6 Pase)mRNA的表达量。结果表明:1)25%糊精组的特定生长率显著高于15%糊精组(P〈0.05)。2)25%糊精组的血清甘油三酯、胆固醇和葡萄糖含量显著高于15%糊精组(P〈0.05)。3)25%糊精组血清己糖激酶(HK)、GK、丙酮酸激酶(PK)、苹果酸脱氢酶(MDH)活性,肝胰脏HK、GK、G6 Pase活性显著高于15%糊精组(P〈0.05),而肝胰脏MDH活性则显著低于15%糊精组(P〈0.05)。4)再投喂24 h后25%糊精组肝胰脏GK mRNA的表达量显著高于15%糊精组(P〈0.05)。再投喂6 h后,25%糊精组G6Pase mRNA表达量显著高于15%糊精组(P〈0.05)。综合分析,在本试验条件下,25%的饲料糊精水平对乌克兰鳞鲤生长和糖代谢的促进作用好于15%的饲料糊精水平。
In order to investigate the effects of dietary dextrin level on growth and carbohydrate metabolism of Ukraine scaly carp (Cyprinus carpio), Ukraine scaly carp with an average body weight about of 3.92 g were used as test object, and 2 experimental diets with 30% protein level were designed which containing 15% and 25% dextrin, respectively. A total of 300 Ukraine scaly carp were randomly divided into 2 groups with 3 repli- cates per group and 50 fish per replicate. After 8 weeks feeding, the growth indices, biochemical indices and carbohydrate metabolize enzyme activities of Ukraine scaly carp were examined. Glucokinase (GK) and glu- cose-6-phosphatase (G6Pase) mRNA expression levels in hepatopancreas and intestine during the refeeding af- ter fasting 48 h were tested. The results showed as follows: 1 ) specific growth rate in the 25% dextrin group was significantly higher than that in the 15% dextrin group (P〈0.05). 2) The contents of serum triglyceride, cholesterol and glucose in the 25% dextrin group were significantly higher than those in the 15% dextrin group (P〈0.05). 3 ) The activities of serum hexokinase ( HK), GK, pyruvate kinase (PK) and malate dehydrogen- ase (MDH) and hepatopancreas HK, GK and G6Pase in the 25% dextrin group were significantly higher than those in the 15% dextrin group (P〈0.05), while the activity of hepatopancreas MDH was significantly lower than that in the 15% dextrin group (P〈0.05). 4) As for the GK mRNA expression level in hepatopancreas at 24 h after refeeding, the 25% dextrin group was significantly higher than the 15% dextrin group (P〈0.05) ; compared with 15% dextrin, the G6Pase mRNA expression level in hepatopancreas was significantly increased by 25% dextrin at 6 h after refeeding (P〈0.05). The results indicate that the improvement effect of 25% of di- etary dextrin level for growth and carbohydrate metabolism of Ukraine scaly carp is better than 15% of dietary dextrin level under this experimental condit