中文摘要：

在那里的背景是在评估的一个困难在里面单个 chondrocytes 的 vivo 功能，并且在骨关节炎( OA )影响的软骨之中有许多异质 .In 这研究，在 vitro ，从改变退化的阶段收获的有教养的 chondrocytes 作为一个射影的模型被学习进一步理解改变结束阶段 OA 的退化的 osteoarthritis.Methods 软骨的致病被收获，当时房间收益和矩阵 glycosaminoglycan (作呕)内容是 measured.Ce

英文摘要：

Background There is a difficulty in evaluating the in heterogeneity among cartilage affected by osteoarthritis vivo functionality of individual chondrocytes, and there is much （OA）. In this study, in vitro cultured chondrocytes harvested from varying stages of degeneration were studied as a projective model to further understand the pathogenesis of osteoarthritis. Methods Cartilage of varying degeneration of end-stage OA was harvested, while cell yield and matrix glycosaminoglycan （GAG） content were measured. Cell morphology, proliferation, and gene expression of collagen type 1,‘11, and X, aggrecan, matrix metalloproteinase 13 （MMP-13）, and ADAMTS5 of the acquired chondrocytes were measured during subsequent in vitro culture. Results Both the number of cells and the GAG content increased with increasing severity of OA. Cell spreading area increased and gradually showed spindle-like morphology during in vitro culture. Gene expression of collagen type II, collagen type X as well as GAG decreased with severity of cartilage degeneration, while expression of collagen type I increased. Expression of MMP-13 increased with severity of cartilage degeneration, while expression of ADAMTS-5 remained stable. Expression of collagen type II, X, GAG, and MMP-13 substantially decreased with in vitro culture. Expression of collagen type I increased with in vitro cultures, while expression of ADAMTS 5 remained stable. Conclusions Expression of functional genes such as collagen type Ⅱ and GAG decreased during severe degeneration of OA cartilage and in vitro dedifferentiation. Gene expression of collagen I and MMP-13 increased with severity of cartilage degeneration.