中文摘要：

目的：建立同时检测Beagle犬血浆中人参皂苷Rg1、Re、Rb1、Rd、Rc、Rb2/3和Rf的分析方法,并应用混合有机溶剂萃取法提取含药血浆中的成分。方法：采用C18反相柱,流动相为甲醇乙腈等比混合液-0.01%甲酸水溶液梯度洗脱,质谱采用多反应监测（MRM）方式进行正离子检测。结果：定量分析的离子对（m/z）为：1131.8/789.5（G-Rb1）、1101.7/789.7（G-Rb2/3）、1101.7/789.7（G-Rc）、823.6/789.5（G-Rd）、969.7/789.7（G-Re）、823.6/643.6（G-Rg1）和823.6/365.3（G-Rf）。7种皂苷类成分定量分析可以在14.5 min内完成,且没有离子抑制现象,各成分的最低定量限分别为0.03125μg.L-（1G-Re和G-Rg1）、0.0625μg.L-（1G-Rf和G-Rd）和0.125μg.L-（1G-Rb1、G-Rc和G-Rb2/3）,该方法的精密度、准确度和稳定性均符合要求。结论：该法选择性强、灵敏度高、操作简便,可用于血浆中7种人参皂苷了成分的药代动力学研究。

英文摘要：

A sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） was developed and validated for the simultaneous quantification of Ginsenoside Rg 1,Re,Rb 1,Rd,Rc,Rb 2/3 and Rf in dog plasma.The compounds were extracted by organic solvent using mixed organic solvent.Chromatographic separation was achieved on a reversed-phase C 18 column with the mobile phase of acetonitrile/menthol-water（0.01% acetic acid） and step gradient elution resulted at a flow rate of 0.23 mL.min-1.A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode.Quantification was performed using multiple reaction monitoring（MRM） of the transitions m/z 1131.8/789.5（G-Rb 1）,1101.7/789.7（G-Rb 2/3）,1101.7/789.7（G-Rc）,823.6/789.5（G-Rd）,969.7/789.7（G-Re）,823.6/643.6（G-Rg 1） and 823.6/ 365.3（G-Rf）.Seven ginsenosides were eluted within 14.5 min and can avoid matrix effect.The quantification limits of the newly developed method were 0.03125 μg.L-1 for G-Re and G-Rg 1,0.0625 μg.L-1 for G-Rf and G-Rd,0.125 μg.L-1 for G-Rb 1,G-Rc and G-Rb 2/3.The precision,accuracy and stability of the analytes meet the requirements.The method was sensitive,accurate and rapid.It can be applied in the determination of plasma concentration of seven ginsenosides.