利用氧化细菌在常温中催化瓦斯气体,并产生溶解氧的特性,采用聚乙烯醇固定化甲烷氧化细菌结合Clark氧化电极技术设计一种新的瓦斯微生物传感器检测方法。选用PVA-海藻酸钠一硼酸交联法固定甲烷的氧化细菌,在磷酸盐缓冲液中催化空气的瓦斯气体,通过Clark氧电极对产生的溶解氧进行电化传输到计算机实时记录。针对传感系统进行瓦斯监测实验表明:微生物传感器实时准确地检测出甲烷气体体积分数,是一种简便,快速,灵敏的瓦斯检测办法。
Make use of the property of oxide bacterium catalyze the gas at room temperature, and produce the dissolving oxygen, polyvinyl alcohol immobilized methane-oxidizing bacteria combined with the Clark oxide electrode technology is used to design a new type of gas microbial sensor method. PVA-alginate-borie acid crosslinking immobilization of methane-oxidizing bacteria is selected. The methane in the air is catalyzed in PBS, DO which is produced through the Oxygen electrode is electrochemicaled and transported to computer to realize realtime record. The experiment which aims at the gas monitoring of the sensor-based system shows that the microbial sensor can detect the volume fraction of methane gas real-time and accurately. It is a kind of simple, swift and sensitive gas-detecting method.