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黄海春季和秋季中华哲水蚤脂类含量及组成分析

ISSN号：0253-4193
期刊名称：海洋学报(中文版)
时间：2014.2.2
页码：99-107
分类：Q948.15[生物学—植物学] S432.42[农业科学—植物病理学;农业科学—农业昆虫与害虫防治;农业科学—植物保护]
作者机构：[1]Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China, [2]University of Chinese Academy of Sciences, Beijing 100049, China, [3]Energy Research Institute, Shandong Academy of Sciences, Jinan 250014, China, [4]State Key Laboratory of Satellite Ocean Environment Dynamics, Secondlnstitute of Oceanography, State OceanicAdministration, Hangzhou 310012, China, [5]Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China, [6]State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China, [7]Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education, Ocean University, of China, Qingdao 266100, China
相关基金：Supported by the National Basic Research Program of China （973 Program）（No. 2011CB409804） and the National Natural Science Foundation of China （No. 41121064） We thank the captain and No. 3 for their guidance crew of the R/V Shiyan and assistance while collecting the samples. We thank Prof. WU Longfei （Laboratoire de Chimie Bacterienne, Universite de la Mediterranee Aix-Marseille II, CNRS, France） for the constructive suggestions.
相关项目：脂类物质在黄海中华哲水蚤种群动力学中的功能作用

关键词：细菌多样性, 群落结构, 中国东海, RRNA基因, 测序法, 16S, 海水样品, 多样性指数, bacterial diversity, community structure, 454 sequencing, water masses, canonical correspondence analysis, East China Sea

中文摘要：

The 454 sequencing method was used to detect bacterial diversity and community structure in the East China Sea. Overall, 149 067 optimized reads with an average length of 454 nucleotides were obtained from 17 seawater samples and fi ve sediment samples sourced in May 2011. A total of 22 phyla, 34 classes, 74 orders, 146 families, and 333 genera were identifi ed in this study. Some of them were detected for the fi rst time from the East China Sea. The estimated richness and diversity indices were both higher in the sediment samples compared with in the seawater samples. All the samples were divided by their diversity indices into four regions. Similarity analysis showed that the seawater samples could be classifi ed into six groups. The groups differed from each other and had unique community structure characteristics. It was found that different water masses in the sampling areas may have had some infl uence on the bacterial community structure. A canonical correspondence analysis revealed that seawater samples in different areas and at different depths were affected by different environmental parameters. This study will lay the foundation for future research on microbiology in the East China Sea.

英文摘要：

The 454 sequencing method was used to detect bacterial diversity and community structure in the East China Sea. Overall, 149 067 optimized reads with an average length of 454 nucleotides were obtained from 17 seawater samples and five sediment samples sourced in May 2011. A total of 22 phyla, 34 classes, 74 orders, 146 families, and 333 genera were identified in this study. Some of them were detected for the first time from the East China Sea. The estimated richness and diversity indices were both higher in the sediment samples compared with in the seawater samples. All the samples were divided by their diversity indices into four regions. Similarity analysis showed that the seawater samples could be classified into six groups. The groups differed from each other and had unique community structure characteristics. It was found that different water masses in the sampling areas may have had some influence on the bacterial community structure. A canonical correspondence analysis revealed that seawater samples in different areas and at different depths were affected by different environmental parameters. This study will lay the foundation for future research on microbiology in the East China Sea.