欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
本文以真核藻类18SrDNA类群特异性PCR引物的设计与评估为例，详细介绍了如何利用Primrose等一系列程序设计类群特异性PCR引物并评估其敏感性与特异性，并对这一方法的优势与应用类群特异性PCR引物进行群落多样性分析需要注意的问题进行了讨论。
英文摘要：
We take the design and evaluation of eukaryotie algae-targeted 18S rDNA PCR primers as an example, to give a step-by-step illustration of how to design and assess group-targeted PCR primers using Primrose and other programs. Then we summarize the advantages of the design ＆ evaluation pipeline by comparing the amplification ef- ficiency of newly designed and previously reported 18S rDNA universal primers. Cautions are also emphasized on u- sing PCR-based strategies to assess community diversity.
同期刊论文项目
同项目期刊论文
Development of 17 novel polymorphic microsatellites in the small yellow croaker Larimichthys polyact
Application of an integrated methodology for eutrophication assessment: a case study in the Bohai Se
Long-term changes in sedimentary diatom assemblages and their environmental implications in the Chan
The migratory history of anadromous and non-anadromous tapertail anchovy Coilia nasus in the Yangtze
Species- and tissue-specific mercury bioaccumulation in five fish species from Laizhou Bay in the Bo
Temporal distribution of bacterial community structure in the Changjiang Estuary hypoxia area and th
Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Parali
Molecular Evidence for Multiple Paternity in a Population of the Viviparous Tule Perch Hysterocarpus
Basin-scale variation in planktonic ciliate distribution: A detailed temporal and spatial study of t
Application of otolith shape analysis for stock discrimination and species identification of five go
Environmental significance of biogenic elements in surface sediments of the Changjiang Estuary and i
Nutrient fluxes in the Changjiang River estuary and adjacent waters---- a modified box model approac
Key nitrogen biogeochemical processes revealed by the nitrogen isotopic composition of dissolved nit
A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique
Geochemical records of decadal variations in terrestrial input and recent anthropogenic eutrophicati
Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in
Fractionation, sources and budgets of potential harmful elements in surface sediments of the East Ch
Air-sea CO2 exchange process in the southern Yellow Sea in April of 2011, and June, July, October of
A numerical study on seasonal variations of the thermocline in the South China Sea based on the ROMS
Survival, growth, food availability and assimilation efficiency of the sea cucumber Apostichopus jap
Elemental signature in otolith nuclei for stock discrimination of anadromous tapertail anchovy (Coil
期刊信息
位置：