中文摘要：

本试验旨在观察磷对体外培养番鸭破骨细胞（OC）生存、生成以及骨吸收功能的影响。分离1日龄番鸭长骨骨髓细胞，培养过程中分别加入不同比例的NaH2PO4、10^-8mol／L1，25-（OH）2D3二因子、不同浓度的NaH2PO4单因子。倒置显微镜观察细胞形态，更换含磷培养液后6h、12h、18h、24h、30h和7d进行抗酒石酸酸性磷酸酶染色（TRAP），分别进行OC计数、形态观察；培养30h内吖啶橙染色，观察OC凋亡情况，7d后扫描电镜观察象牙片吸收陷窝情况。结果表明：在同一时间点，添加磷培养液均能抑制1，25-（OH）2D3诱导产生OC，并抑制OC的骨吸收活性，抑制程度与磷浓度成正比，3、5和7mmol／L组OC数量均极显著少于对照组（P〈0．01）；5mmol／L组OC数量极显著少于3mmol／L组（P〈O．01）；5mmol／L组与7mmol／L组差异不显著；更换含磷液培养12、18、24和30h，均可见磷含量达3mmol／L时OC数量均极显著少于对照组（P〈0．01），磷浓度相同组，OC数量随着培养时间的延长而减少。添加磷培养液能够抑制体外诱导培养OC的生成及骨吸收活性，抑制体外培养成熟OC的生存。

英文摘要：

The experiment was conducted to study the effects of phosphorus on the formation and resorption activity of muscovy duck＇s osteoclasts （OC） induced from bone marrow mesenchymal stem cells in vitro. Osteoclasts were isolated from long bones of 1 day old muscovy duck and cultured with different concentrations of Nail2 PO4 and different concentrations of Nail2 PO4 containing 10 s mol/L 1,25-（OH） 2 D3, respectively. The tartrate-resistant acid phosphatase （TRAP） staining was carried out 6 h, 12 h, 18 h, 24 h, 30 h and 7 d after replacement of culture liquid with different concentrations of phosphorus, respectively, to count the number and observe the shape of TRAP positive OC, and acridine orange staining was carried out to observe the apoptosis of OC within 30 h, and lacunar resorption was detected on the 7th day through scanning electron microscopy. The result showed that adding phosphorus could inhibit the formation and bone resorption activity of OC induced from muscovy duck＇s bone marrow mesenchymal stem ceils with 1,25-（OH）2 D3 at the same time point, and the inhibition degree was direct proportional to the Nail2 PO4 concentration from 3 mmol/L to 7 mmol/L. The number of OC coultured with phosphorus （≥3 mmol/L） was significantly smaller than that of control group （P〈0.01）. The number of OC in 5 mmol/L group was significantly smaller than that in 3 mmol/L group （P〈0.01） and there was no significant difference between 5 mmol/L group and 7 mmol/L group. In addition, 6, 12, 18, 24 and 30 h after culture liquid replaced by adding different concentrations of phosphorus, the number of OC co-cultured with phosphorus （≥3 mmol/L） was significantly smaller than control group （P〈0.01）, and the number of OC in group with the same concentration group was decreasing with the prolongation of time. In a word, phosphorus could inhibit the formation and resorption activity of OC and induce apoptosis of OC in vitro.