中文摘要：

目的研究甲源性念珠菌的菌种分类和药敏结果,并对咪康唑敏感性不同的白念珠菌和近平滑念珠菌株的基因型进行聚类分析。方法采用ROSCO抗真菌药敏纸片对念珠菌菌株进行药敏分析;ERIC-PCR指纹法分别比较白念珠菌及近平滑念珠菌的咪康唑敏感株,中敏株及耐药株的基因型,并对其聚类分析。结果白念珠菌和近平滑念珠菌对咪康唑的敏感性分别为42%和32%。而对咪康唑敏感性不同的白念珠菌以及近平滑念珠菌的ERIC-PCR指纹图谱未见特异性条带。结论 ERIC-PCR指纹法可用于不同念珠菌菌株的基因分型;但其念珠菌的基因分型与咪康唑耐药无一定相关性。

英文摘要：

Objective To study strain classification and drug sensitivity of Candida in onychomycosis,and to clusteringly analysize the genotypes of C.albican and C.parapsilosis which are differently sensitive to miconazole.Methods Drug sensitivity of Candida were examined with ROSCO antifungal paper.Genotype analysis of C.albican and C.parapsilosis sensitive,medisensitive or resistant to miconazole was performed by ERIC-PCR.Results Drug sensitivity rates of C.albican and C.parapsilosis were 42% and 32% respectively.No specific DNA profiles of C.albican and C.parapsilosis were identified.Conclusions ERIC-PCR method could be applied in genotype analysis for different strains of Candida.Genotype of Candida has no relationship with resistance to miconazole.