中文摘要：

目的：探讨补肾益髓生血法对苯与环磷酰胺（CTX）诱导再生障碍性贫血（AA）大鼠免疫及造血功能的影响。方法：雄性SD大鼠85只,随机分为正常组10只,造模组75只。造模组大鼠皮下注射苯（1mL/kg）与腹腔注射CTX（25mg/kg）,模型成功后随机分为模型、康立龙、益髓生血、温肾生血和滋肾生血5组。7周末处死大鼠,检测血常规;流式细胞术检测CD3、CD4、CD8;血涂片、骨髓涂片瑞氏染色,观察血、骨髓细胞形态;检测骨髓悬液骨髓有核细胞数;脾组织行HE染色,观察病理形态。结果：与模型组比,各治疗组红细胞（RBC）、白细胞（WBC）、血红蛋白（HGB）均显著性增加（P〈0.05,P〈0.01）,CD8显著降低（P〈0.05,P〈0.01）,CD4、CD4/CD8有增加趋势,CD3有降低趋势;模型组血涂片红细胞呈散状分布、有核细胞减少,骨髓涂片脂肪滴明显增多,有核细胞增生降低等,各治疗组均明显好转;与模型组比,各治疗组骨髓有核细胞明显增加（P〈0.01）;模型组脾淋巴结弥散,红髓和白髓边界不清,各治疗组淋巴结趋于正常,红髓白髓边界趋于清晰。结论：补肾益髓生血法可能通过调控AA免疫功能,减轻对骨髓造血功能的病理损害,增加外周血细胞及淋巴细胞,进而起到显著的治疗作用,滋肾生血法明显优于益髓生血法和温肾生血法。

英文摘要：

Objective： To explore the influence of Bushen Yisui Shengxue treatment on the function of medullary hematopoiesis and immunity of aplastic anemia（AA） rats induced by benzene and cyclophosphamide（CTX）. Methods： Eightyi ve male SD rats were randomly divided into normal group（10 rats） and molding group（75 rats）. Rats in the molding group were given subcutaneous injection with benzene（1mL/kg） and intraperitoneal injection with CTX（25mg/kg）. After the AA rats model was established, rats in the molding group were randomly divided into model group, stanozolol group, Yisui group, Wenshen group and Zishen group. All the rats were sacrii ced in the seventh week. The blood samples of femoral artery were tested by the blood routine examination. The l ow cytometry（FCM） was used to test the CD3, CD4, and CD8. The blood smear, bone marrow smear and wright＇s staining were used to test the morphology of blood cells, bone marrow cell and the nucleated cells of the bone marrow suspension. HE stain was used to test the pathological morphology of the spleen. Results： Compared with the model group, the RBC, WBC and HGB in each treatment group were signii cantly increased（P〈0.05, P〈0.01）. Compared with the model group, CD8 in each treatment group were reduced（P〈0.05, P〈0.01）, CD3 in each treatment group had reduced trend, and the CD4 and CD4/CD8 in each treatment group had increased trend. The red cells in the model group were scattered and the nucleated cells reduced. The fat droplets of bone marrow smear were increased signii cantly, and the hyperplasia of nucleated cells was decreased. Compared with the model group, the nucleated cells of marrow in each treatment group were increased（P〈0.01）. The splenic lymph nodes of rats in the model group were scattered, and the border of red pulp and white pulp was not obvious, while the indexof treatment groups returned to normal. Conclusion： Bushen Yisui Shengxue treatment could relieve the pathological lesion on the bone marrow hemo