中文摘要：

目的：建立同时测定敷胸巴布贴中5种大黄蒽醌类成分（芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）含量的方法。方法：采用Agilent TC-C18（250 mm×4.6 mm,5μm）色谱柱,流动相甲醇-0.4%磷酸溶液（85：15）,流速1.0 m L·min-1,柱温30℃,紫外检测波长254 nm。结果：5种蒽醌类成分之间有较好的分离度,芦荟大黄素线性范围0.094 2-0.942μg（r=0.9995）,大黄酸线性范围0.036 9-0.369μg（r=0.9996）,大黄素线性范围0.048 6-0.486μg（r=0.9999）,大黄酚线性范围0.032 4-0.324μg（r=0.9998）,大黄素甲醚线性范围0.069 6-0.696μg（r=0.9997）,平均加样回收率分别为98.7%,98.3%,96.8%,96.8%,97.4%,RSD分别为0.7%,1.0%,2.1%,1.8%,2.4%。结论：该实验方法精确度高、重复性好,可用于敷胸巴布贴的质量控制。

英文摘要：

Objective：To establish the method for simultaneous determination of five Rheum offieinale anthraquinones （including aloeemodin, rhein, emodin, chrysophanol, physcion） from Fuxiong Cataplasms. Methods：The analysis was achieved with an Agilent Zorbax TC -Ctsanalytical column（4.6 mm × 250 ram,5 μm） used with the column temperature 30 ℃. The detection wavelength for UV detector was 254 nm. The flow rate was set at 1.0 mL · min- 1 and mobile phase was consisted of methanol - 0.4% phosphoric acid（ 85 ： 15 ）. Results ： All the active constituents were well separated by this method. Linearities of aloe - emodin, rhein, emodin, chrysophanol and physcion were good in the ranges of 0.0942 - 0. 942 μg （ r = 0. 9995 ）, 0. 0369 - 0. 369 μg （ r = 0. 9996） ,0. 0486 - 0. 486 μg（ r = 0. 999 9） ,0.0324 - 0. 324 μg（ r = 0. 999 8） ,0. 0696 - 0. 696 μg（ r = 0.9997 ）, respective- ly. The average recoveries of aloe - emodin, rhein, emodin, chrysophanol and physcion were 98.7 % ,98.3 % ,96.8 % ,96.8 % and 97.4% and RSD values were 0.7%, 1.0% ,2.1%, 1.8% ,2.4%. Conclusion：This method is accuracy and reproducibility. It can be effectively used for the quality control of Fuxiong Cataplasms.