中文摘要：

目的：建立HPLC法测定敷胸膏中黄芩苷、大黄酚、大黄素、大黄素甲醚、芦荟大黄素、大黄酸的含量。方法：采用高效液相色谱法。色谱条件为Agilent TC-C18（4.6 mm×250 mm,5μm）色谱柱,乙腈—0.05%磷酸水溶液为流动相;检测波长：254 nm。柱温：30℃;流速：1.0 m L/min。结果：黄芩苷、大黄酚、大黄素、大黄素甲醚、芦荟大黄素、大黄酸分别在0.1700~4.2500μg、0.0180~0.4500μg、0.0255~0.6375μg、0.0387~0.9675μg、0.0052~0.1308μg、0.0205~0.5125μg范围内线性关系良好（r分别为0.9998、0.9999、0.9999、0.9999、0.9997、0.9998）;平均加样回收率分别为97.57%、98.29%、98.08%、98.01%、97.78%、97.44%;RSD分别为2.01%、1.37%、1.86%、1.63%、1.35%、1.53%。结论：该方法可用于测定黄芩苷、大黄酚、大黄素、大黄素甲醚、芦荟大黄素、大黄酸的含量,方法操作简便、准确、重复性好,为复方质量标准评价提供了依据。

英文摘要：

Objective： The HPLC method was developed to determine baicalin,rhubarb phenol,emodin,emodin methyl ether,aloe emodin and rhein in Fuxiong Plaster. Methods： The HPLC analysis was carried out on Agilent TC- C18column（ 4. 6 mm ×250 mm,5 μm）,acetonitrile- 0. 05 mol / L（ NH4）2HPO4was used as the mobile phase. The detection wave length was at 254 nm and the flow ratew as 1 m L / min. Results： The linear ranges of baicalin,rhubarb phenol,emodin,emodin methyl ether,aloe emodin and rhein were 0. 1700- 4. 2500 μg,0. 0180- 0. 4500 μg,0. 0255- 0. 6375 μg,0. 0387- 0. 9675 μg,0. 0052- 0. 1308 μg and0. 0205- 0. 5125 μg which indicated the linearity of this method was all right,and the r values were 0. 9998,0. 9999,0. 9999,0. 9999,0. 9997 and 0. 9998. The average recoveries of them were 97. 57%,98. 29%,98. 08%,98. 01%,97. 78% and 97. 44%and the RSD values were 2. 01%,1. 37%,1. 86%,1. 63%,1. 35% and 1. 53%. Conclusion： This method can be applied in quantitative determinations of baicalin,rhubarb phenol,emodin,emodin methyl ether,aloe emodin and rhein in Fuxiong Plaster with a good reproducibility. It can be used as an analysis method for compound quality standard.