中文摘要：

目的：探索调气活血法治疗大鼠慢性萎缩性胃炎的疗效及其对胃酸分泌影响的机制。方法：采用N-甲基-N-硝基-亚硝基胍（MNNG）自由饮用的综合造模法复制大鼠慢性萎缩性胃炎动物模型,造模成功后,分别给予调气活血方、胃复春片、蒸馏水灌胃治疗8周,处死大鼠,收集大鼠胃酸,测胃酸p H值,取大鼠胃组织,苏木素-伊红染色（HE染色）观察病理变化,免疫组织化学方法检测胃黏膜质子泵（H,K-ATPase）、ezrin蛋白表达水平。结果：调气活血方可有效治疗大鼠慢性萎缩性胃炎,显著提高大鼠胃酸分泌水平,增加胃黏膜H,KATPase、ezrin蛋白的表达面积,与空白模型组和胃复春组比较,差异具有统计学意义（P〈0.01,P〈0.05）。结论：调气活血法可能通过提高胃黏膜ezrin蛋白表达水平,促进了H,K-ATPase在壁细胞顶膜锚定,从而促进了壁细胞酸分泌,达到治疗大鼠慢性萎缩性胃炎和延缓疾病进展的作用。

英文摘要：

Objective： To explore the effect and gastric acid secretion mechanism of Tiaoqi Huoxue treatment on chronic atrophic gastritis rats. Methods： Chronic atrophic gastritis rat model was induced by a comprehensive method based on MNNG free drinking. Tiaoqi Huoxue Decoction, Weifuchun Pill and distilled water were respectively treated orally for 8 weeks. Then all rats were sacrificed. Gastric juice were collected and tested by p H-value measurement. Stomachs were collected and dyed with Hematoxylin and eosin（HE） to observe pathology. Immunohistochemistry was employed to detect the expression of H,KATPase and ezrin of gastric mucosa. Results： Tiaoqi Huoxue Decoction had a therapeutic effect on rats with atrophic gastritis. Compared with the model group and Weifuchun group, the Tiaoqi Huoxue Decoction raised the amount of gastric acid secretion and increased the expression of H,K-ATPase and ezrin in rats＇ gastric mucosa with statistically significant（P〈0.01, P〈0.05）. Conclusion： Tiaoqi Huoxue treatment could raise the amount of acid secretion of parietal cell and slow down the progression of chronic atrophic gastritis of rats by improving the expression of ezrin in gastric mucosa, promoting proton pump H,K-ATPase docking at the apical membrane of parietal cell.