中文摘要：

目的建立川芎嗪长循环脂质体的包封率测定方法。方法采用葡聚糖凝胶-G50微型柱分离脂质体和游离药物，以高效液相色谱法分别测定含量，并计算包封率。结果葡聚糖凝胶-G50可使川芎嗪长循环脂质体和游离药物完全分离；HPLC条件下，川芎嗪与辅料和溶剂峰分离良好，川芎嗪浓度在1．09～43．60[zg／mL范围内线性关系良好（r=0．9994，n=6）。结论所建立的方法简单可行、重复性好、准确、稳定，为评价和控制川芎嗪长循环脂质体的质量提供了依据。

英文摘要：

Objective To establish a method for determination of the entrapment efficiency of Tetramethylpyrazine long-circulating liposomes. Methods The Tetramethylpyrazine long-circulating liposomes and free Tetramethylpyrazine were separated by Sephadex G-50 chromatography, and quantified by HPLC. Results The Tetramethylpyrazine longcirculating liposomes and free Tetramethylpyrazine were well separated by Sephadex G-50 chromatography; it was found that the excipients in the liposome did not interfere with the assay. Tetramethylpyrazine had a good linearity over the range of 1.09-43.60 μg/mL （r = 0.9994, n = 6）. Conclusion This method is simple, accurate and sensitive for the quality control of the Tetramethylpyrazine long - circulating liposomes, and can provide evaluation and control for the quality of Tetramethylpyrazine long circulating liposomes.