中文摘要：

目的克隆人AnnexinⅡ全长cDNA序列，并构建其真核表达载体。方法根据Genbank中AnnexinⅡ基因的碱基序列，利用RT—PCR的方法从人肾脏组织中扩增编码AnnexinⅡ基因，将目的片段与pBS—T载体连接，利用亚克隆的方法将AnnexinⅡ的cDNA片段克隆到peDNA3．1载体中，并进行序列测定。结果DNA测序证实该片段序列与Genbank完全一致，结论成功克隆人AnnexinⅡ全长cDNA序列，并构建出peDNA3．1-AnnexinⅡ真核表达载体，为进一步研究其在肾脏疾病中的作用和机制奠定基础。

英文摘要：

To clone human full length annexin Ⅱ gene and construct recombinant eukaryotic expression vector containing annexin Ⅱ gene, according to the gene sequence in Genbank, the annexin Ⅱ cDNA was obtained from human kidney tissue by RT- PCR. The target gene was cloned into pBS -T vector, then subcloned into pcDNA3.1 expression vector. Sequencing was employed to identify the correct clones. The sequence result showed the human annexin Ⅱ gene is correct according to the reports. Human full length annexin Ⅱ gene was successfully cloned and the recombinant pcDNA3. 1 - annexin Ⅱ eukaryotic expression vector was constructed. These pave the way for understanding its role and mechanisms in kidney disease.