中文摘要：

目的：观察霉酚酸酯（MMF）对多囊肾囊肿衬里上皮细胞增殖和凋亡的影响，并与雷帕霉素（RAPA）比较。方法：采用原代培养的人多囊肾囊肿衬里上皮细胞，用不同浓度MMF和RAPA分别作用48h和72h。MTT法检测细胞增殖情况；流式细胞术检测细胞周期变化；Annexin、V-FITC流式细胞术测定细胞凋亡率；透射电镜观察细胞超徽结构改变。结果：MMF和RAPA均能抑制囊肿衬里上皮细胞增殖，呈明显的量效和时效关系。作用48h后MMF使细胞阻滞在S期，RAPA使细胞阻滞在G0／G1期。MMF和RAPA均可诱导细胞凋亡，最大凋亡率分别为（5．53±0．27）％和（4．36±0．10）％；电镜下可见典型的凋亡改变。结论：MMF与RAPA相比同样能明显抑制囊肿衬里上皮细胞增殖，诱导细胞凋亡，但其对细胞增殖的抑制作用较RAPA弱。

英文摘要：

Objective：To investigate the effect of mycophenolate mofetil（MMF）on proliferation and apoptosis of cyst-lining epithelial cells in patients with autosomal dominant polycystic kidney disease （ADPKD）, and to compare its effect with that of rapamycin （RAPA）in vitro. Methods： Primary cultured cyst-lining epithelial cells were treated with MMF and RAPA at different concentrations（0,0. 005,0.05,0.5,5 μg/ml）for 48 h or 72 h. The inhibitory effects of them on the cells were evaluated by MTT assay; the cell cycle distribution and apoptotic ratio were determined by flow cytometry. The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope. Results： Both MMF and RAPA significantly inhibited the proliferation of cyst-lining epithelial cells in a dose- and time-dependent manner. After 48 h treatment,the cells were blocked at S phase by MMF and at G0/G1 phase by RAPA. Both drugs induced cell apoptosis,with the maximal apoptotic rate being （5. 53 ± 0. 27）% for MMF and （4. 36 ± 0. 10）% for PAPA. Typical morphological changes of apoptotic cells were observed under electron microscope. Conclusion： MMF can effectively inhibit proliferation and induce apoptosis of cyst-lining epithelial cells, but its inhibitory effect is weaker than that of RAPA.