中文摘要：

目的：比较多囊蚤白-1在肾透明细胞癌、常染色体显性遗传多囊肾病患者及正常人体液中的含量。方法：随机选择在本院就诊的肾透明细胞癌患者20例、常染色体显性多囊肾病患者20侧及正常健康查体者15例，留取血液及尿液标本各1份。以本实验室制备的鼠源抗多囊蛋白-1单克隆抗体为捕获抗体，以免源抗多囊蛋白1多克隆抗体为检测抗体，采用夹心酶联免疫吸附试验（ELISA）定量检测体液中多囊蛋白1的含量。结果：肾透明细胞癌患者血液中多囊蛋白1含量为（53．613±8．697）μg/L，显著高于多囊肾病患者血液中含量（6.745±3.215）μg/L（P〈0．01）及正常人血液中含量（20．386±14．234）ng／ml（P〈0．01）。肾透明细胞癌患者尿液中多囊蛋白-1含量为（10．228±7．989）μg/L，显著低于多囊肾病患者尿液中含量（48．268±16，452）ng／ml（P〈0．01），与正常人尿液中含量（10．0813±8．941）μg/L较无统计学差异。结论：多囊蛋白-1属于一个新的跨膜蛋白家族，在肾透明细胞癌患者血液中表达增加，而在多囊肾患者血液中表达减少，为进一步揭示多囊蛋白-1的功能提供实验室依据。

英文摘要：

Objective： To comparatively study on polycystin- 1 in the body fluid of the patients of clear cell renal carcinoma and autosomal dominant polyeystie kidney disease. Methods：20 cases of clear cell renal carcinoma, 20 ease of ADPKD and 15 cases of normal control were observed in this study. Every patient collected one blood sample and one urine sample respectively, We established a .sandwich ELISA method, The polycystin - 1 in the serum and urine of clear cell renal carcino ma patients, ADPKD patients and the normal controls were detected. Results： The quantitative analysis revealed that the polycystin-1 in the serum of clear cell renal carcinoma, which was （53.613 ± 8. 697）μg/L, was markedly higher than that in ADPKD and the normal control, which were （6. 745 ±3. 215）μg/L and （20. 386±14. 234 ）μg/L respectively. （P〈0.01） The polycystin- 1 in the urine of clear cell renal carcinoma, which was （10. 228 ± 7. 989）μg/L, was markedly lower than that in ADPKD, which was （48. 268 ± 16. 452）μg/L（P 〈 0.01）. Conclusion： The polycystin- 1 was markedly higher in the serum of the patients of clear cell renal carcinoma, compared with that in ADPKD and normal controls. Data of this study may provide, at least partly, valuable experimental evidence of the function of polycycstin - 1.