中文摘要：

目的探讨GRIM-19对宫颈癌细胞株HeLa增殖行为的影响及其机制。方法采用RT-PCR和Western blot检测基因的mRNA和蛋白水平表达情况。将HeLa/control、HeLa/GRIM-19接种到裸鼠皮下检测荷瘤情况,利用免疫组织化学法检测Ki-67的表达。结果 HeLa/GRIM-19细胞接种肿瘤生长速度明显减慢（P〈0.01）。移植瘤的RT-PCR结果：GRIM-19表达升高、而其下游基因STAT3、Cyclin B1、Bcl-L2的表达降低;蛋白检测发现STAT3、p-STAT3、suvivin、Ki-67基因的表达均下降,GRIM-19表达升高。结论 GRIM-19的表达可以抑制宫颈癌细胞的增殖,可能是宫颈癌治疗的新靶点。

英文摘要：

Objective To explore the effects of GRIM-19 expression on the growth of cervical cancer in vivo and its mechanisms.Methods GRIM-19 gene expression was investigated at mRNA and protein levels by RT-PCR and Western blot.Tumor growth of the null mice was analyzed after injection HeLa/control,HeLa/GRIM-19 into the skin.The protein level of Ki-67 was inspected with immumohistochemistry methods.Results The growth of HeLa/GRIM-19 implanted tumor slowed down significantly（P0.01）.The expression of GRIM-19 mRNA was up-regulated,while STAT3,cyclin B1,Bcl-L2 mRNA were decreased in tumors bearing HeLa/GRIM-19.The protein level of STAT3,p-STAT3,suvivin,ki-67 was reduced too; while the expression of GRIM-19 was increased by protein test.Conclusion The expression of GRIM-19 can slow down the proliferation of cervical cancer cells,suggesting that GRIM-19 may be a new target for the treatment of cervical cancer.