中文摘要：

以烟草悬浮细胞BY~（-2）为材料,利用FoF1-ATP合成酶（FoF1-ATPase）抑制剂寡霉素研究了细胞内ATP（i ATP）对胞外ATP（e ATP）水平的影响,以及施加外源ATP对i ATP水平和细胞死亡的调节作用。结果表明,随着寡霉素浓度的增加（5、10、25、50μmol·L~（-1））,烟草悬浮细胞的i ATP含量逐渐下降,e ATP含量也随之减少,且细胞死亡水平在较高寡霉素处理浓度下有明显上升。同时,随着寡霉素处理时间的增加（0.5、1、3、5 h）,烟草悬浮细胞的i ATP含量和e ATP含量也呈现出不同程度的下降,而细胞死亡水平则有所增加。施加外源ATP能够缓解寡霉素引起的细胞i ATP水平的下降和细胞死亡水平的上升。上述结果表明,e ATP水平受到了i ATP水平的影响,而e ATP也在线粒体ATP合成受到抑制时调控i ATP和细胞死亡的发生。

英文摘要：

In BY-2） tobacco suspension cells,the effect of intracellular ATP（ i ATP） production on extracellular ATP（ e ATP） levels was studied by using oligomycin（ Inhibitors of F_oF_1-ATPase）. The results showed that with the increase of the concentrations of oligomycin（ from 5- 50 μmol·L~（-1））,the level of i ATP and e ATP were decreased,and the level of cell death were increased under higher concentration of oligomycin. With the increase of the times of oligomycin treatment（ 0. 5,1,3 and 5 h）,the levels of both i ATP and e ATP were decreased in different extents,while the level of cell death were increased. Treatment with exogenous ATP alleviated the decrease in i ATP and the increase in cell death level that were caused by oligomycin,The level of e ATP can be affected by i ATP,and e ATP can regulate the i ATP production and cell death under the condition of mitochondrial oxidative phosphorylation being inhibited.