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Capillary Electrophoresis for the Simultaneous Determination of Metals by Using Ethylenediamine Tetraacetic Acid as Complexing Agent and Vancomycin as Complex Selector

ISSN号：1001-604X
期刊名称：《中国化学：英文版》
时间：0
分类：O657.8[理学—分析化学;理学—化学]
作者机构：[1]The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Department of Chemistry, Tsinghua University, Beijing 100084, China, [2]Department of Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand
相关基金：Project supported by the National Natural Science Foundation of China （Nos. 20437020 and 20575008） and the Post Doctoral Research Scholarship of Department of Chemistry, Tsinghua University.

作者： THREEPROM Jirasak[1,2], SOM-AUM Waraporn[1], 林金明[2]

关键词：毛细管电泳分析, 万古霉素, 金属离子, 同时测定, 乙撑二胺四乙酸, Z-shape cell capillary electrophoresis, vancomycin, metal ion, ethylenediamine tetraacetic acid

中文摘要：

为由把 ethylenediamine tetraacetic 酸(EDTA ) 用作复杂选购者被描述的 complexing 代理人和采用的万古霉素的金属的同时的决心的毛状的电气泳动的一个新分离系统。Z 形状房间毛状的电气泳动被用来为建筑群的决心提高敏感 Cu (II ) ， Ni (II ) ，公司(II ) 和有 EDTA 的 Fe (III ) 。部分充满方法(合作水流模式) 被使用以便增加 electrophoretic 方法的选择，同时万古霉素不在 metal-EDTA 建筑群的察觉期间是在察觉者路径的现在。万古霉素集中，磷酸盐集中和缓冲区的 pH 强烈影响了学习 analytes 的活动性，分辨率和选择。在最佳的条件下面，迁居时间和山峰区域的相对标准差(n=5 ) 分别地是不到 3.14% 和 7.35% 。Z 形状房间的申请有装载的紫外察觉和万古霉素的毛状的电气泳动方法在自来水和恢复 were97%101% 导致了这些金属离子的可靠决心。基于到噪音的一个信号， 3:1 的比率在 210 g explor 砌 ? 砌的范围被发现的察觉限制？？

英文摘要：

A new separation system of capillary electrophoresis for the simultaneous determination of metals by using ethylenediamine tetraacetic acid （EDTA） as complexing agent and employing vancomycin as complex selector was described. The Z-shape cell capillary electrophoresis was used to enhance the sensitivity for the determination of the complexes of Cu（Ⅱ）, Ni（Ⅱ）, Co（Ⅱ） and Fe（Ⅲ） with EDTA. The partial filling method （co-current mode） was used in order to increase the selectivity of the electrophoretic method, meanwhile vancomycin was not present at the detector path during the detection of metal-EDTA complexes. The vancomycin concentration, phosphate concentration and pH of the buffer strongly influenced mobility, resolution and selectivity of the studied analytes. Under the optimal condition, the relative standard deviations （n=5） of the migration time and the peak area were less than 3.14% and 7.35%, respectively. Application of the Z-shape cell capillary electrophoresis method with UV detection and vancomycin loading led to the reliable determination of these metal ions in tap water and the recoveries were 97%-101%. The detection limits based on a signal to noise ratio of 3 ： 1 were found in the range of 2-10 μg·L^-1.