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中文摘要：
利用化学发光免疫分析（chemiluminescence immunoassay，CLIA）高灵敏度和高特异性的特点，将磁性微粒子应用于化学发光免疫分析中，用两种不同的方法对人体血清内的雌三醇（E3）含量进行了测定．磁性微粒子分别作为固相一抗包被材料和二抗分离剂参与反应．两种方法检测雌三醇浓度的线形范围均为0．6～60ng／mL．其中，固相-抗法的批内变异及批间变异系数分别小于11％和15％，回收率为90％116％，健全性系数为0．9987．二抗分离法的批内变异及批间变异系数分别小于8％和10％，回收率为88％～118％，健全性系数为0．9974、两种方法分别与经典板式化学发光法对比，检测人血清样本，结果相关性较好，且磁性微粒子法更为省时、简便，适于推广应用．
英文摘要：
A chemiluminescence immunoassay （CLIA） method of high sensitivity, selectivity and reproducibility for the determination of estriol （E3） in human serum was developed. The magnetic microparticles were used as the second-antibody separation agent and the first-antibody solid-surface, respectively. Under the selected conditions, the two methods with magnetic microparticles as the second-antibody separation agent and the first-antibody solid-surface respectively, showed a calibration curve for E3 both in the range of 0.6-60 ng/mL. In the first-antibody method, the CVs （coefficient of variations） of intra- and inter-assays were less than 11% and 15%, respectively. The recovery was in the range of 90%N 116%, and the soundness coefficient was 0.9987. In the second-antibody method, the CVs of intra- and inter-assays were less than 8% and 10%, respectively. The recovery was in the range of 88%-118%, and the soundness coefficient was 0.9974. Compared with the traditional micro-plate CLIA method, both of the proposed methods exhibited less analysis time and labor. The proposed methods have been used to determine E3 in human serum successfully.
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