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中文摘要：
目的研究全反式维甲酸（ATRA）作用于人白血病细胞系U937细胞后，对细胞生长的影响及可能的机制。方法收集1 μmoL／L ATRA作用后的U937细胞，利用流式细胞术检测细胞周期分布，Western blot法检测细胞周期相关蛋白（cyclinA、p16、p21、p27及p27相关分子skp2）表达水平的变化，采用免疫沉淀方法检测U937细胞中p27与Skp2的结合情况。结果流式细胞术分析显示，在1 μm0L／L ATRA作用72h后，72％的U937细胞被阻滞在G0／G1期。Western blot分析显示，ATRA能诱导cyclinA、Skp2表达下凋，p21、p27表达上调。免疫沉淀法检测结果显示在U937细胞中p27与Skp2存在相互结合的情况。结论ATRA可能主要通过诱导p27表达上调引起U937细胞生长阻滞，其过程是由p27相关分子Skp2所介导的。
英文摘要：
Objective To study the effect of all-trans retinoic acid （ATRA） on U937 cell growth and its mechanism. Methods Cell cycle was detected by flow cytometry （ FCM）, expressions of cell cycle associated protein and the p27 related protein were detected by Western blot. The binding of P27 and Skp2 was detected by immunoprecipitation. Results FCM displayed that ATRA could inhibit the proliferation of U937 cells. At 72 h on 1 μmol/L ATRA treatment, 72% of the cells were arrested at G0/G1 phase. Western blot displayed that ATRA could decrease the expression of cyclinA, up-regulate the expression of p21 and p27, and down-regulate the expression of p27 related proteins Skp2. p27 could bind with Skp2 in U937 cells as detected by immunoprecipitation. Conclusion ATRA may arrest the proliferation of U937 cells through the reduction of Skp2 expression, and finally the induction of the accumulation of p27.
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