中文摘要：

目的 研究细菌脂多糖（LPS）对培养的大鼠星形胶质细胞中Src抑制的蛋白激酶C的底物（SSeCKS）表达的影响。方法 培养的星形胶质细胞随机分为空白对照组、LPS单一刺激组、LPS联合PKC抑制剂（RO-31-8220）刺激组。运用荧光定量PCR（Real time RT-PCR）、免疫印迹和免疫细胞化学法分析SSeCKS的表达变化和亚细胞定位。结果 Real time RT-PCR显示，LPS可以上调SSeCKS mRNA水平，在作用浓度为100μg／L和1mg／L时与对照组有显著差异（P〈0．01）。Western blotting表明，当LPS作用浓度为100μg／L时，SSeCKS蛋白表达量明显上调；在此浓度作用下，SSeCKS表达于6h达高峰并广泛磷酸化，至24h其蛋白表达仍维持于较高水平。免疫细胞化学分析显示，正常情况下，SSeCKS散在分布于胞质，于胞膜略有浓集。在LPS单一刺激组，SSeCKS富集于核周；当LPS联合RO-31-8220共同作用时，SSeCKS的亚细胞定位与正常组无明显差异。结论 在体外培养的星形胶质细胞中，LPS可诱导SSeCKS表达，上调其磷酸化水平，影响其细胞内定位。这些改变与PKC的功能相关。提示SSeCKS可能参与星形胶质细胞中炎症信号的转导。

英文摘要：

Objective To investigate the effects of lipopolysaccharide （LPS） on Sre-suppressed C Kinase Substrate （SSeCKS） in euhured astroeytes. Methods Purified astrocytes were randomly divided into three groups： control group, singly stressed and multiple stressed groups. The expression of SSeCKS was detected by Real time RT-PCR and Western blotting, while immunocytochemistry was used to investigate the subeellular localization of it. Results Real time RT-PCR indicated that, after 12 hours incubation, both 100 μg/L and 1mg/L LPS were able to elevate the levels of SSeCKS mRNA compared with control group, while 1mg/L LPS did not have a stronger effect than 100 μg/L. Western blotting analysis showed 100 μg/L LPS significantly increased the expression of SSeCKS. In time response experiments, the levels of SSeCKS expression enhanced three hours after the stimulation, peaked at the sixth hour, coincident with its rapid phosphorylation, and remained high until the 24th hour. Immunocytochemistry suggested a perinuelear transloeation of SSeCKS, while the PKC inhibitor RO-31-8220 blocked it. Conclusion In cultured astroeytes, LPS can enhance the expression of SSeCKS, increase its phosphorylation level and change its subeellular localization. These effects are correlated with the PKC pathway, which indicates SSeCKS might participate in the signal transduction of inflammation in cuhured astrocytes.