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中文摘要：
目的 探讨正常及损伤的周围神经中神经型一氧化氮合酶（nNOS）相关分子（NIDD）的表达定位与变化。方法 在利用实时荧光定量PCR（FQ-PCR）研究大鼠坐骨神经损伤对nNOS及其相关分子NIDD mRNA表达变化的基础上，通过原位杂交与间接免疫荧光相结合的方法进一步研究其表达定位情况。结果 nNOS及NIDD mRNA主要分布在正常和损伤坐骨神经的施万细胞（SCs）中，损伤后表达增多，分别在神经夹伤后2周以及切断后1周的近、远侧断端中出现表达高峰。原代培养的SCs中也检测到nNOS及NIDD，其mRNA分布在核周细胞质区域。结论 周围神经损伤对nNOS相关分子NIDD的表达有影响，SCs表达NIDD可能参与神经损伤后再生修复机制。
英文摘要：
Objective To study the localizations and changes of mRNA for nNOS-interacting DHHC domain-containing protein with dendritic mRNA （NIDD） in normal and injured peripheral nerves. Methods Real-time tluorescence quantitative PCR （FQ-PCR） and a combination of in situ hybridization and indirect immunotluorescence were used to detect the localizations and changes of mRNA for nNOS and NIDD. Results It was found that nNOS and NIDD were mainly localized in Schwann cells （SCs） of normal sciatic nerve, and the expression peaked in crushed sections of sciatic nerve in the 2nd week after injury and in proximate or distal transected stumps in the 1st week after injury respectively. Expressions of mRNA for nNOS and NIDD were detected in the perinuclear plasm of primary SCs as well. Conclusion The increase in mRNA for NIDD in SCs may play an important role in axonal regeneration after nerve injury.
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