为了探明乌鸡中枢神经系统内神经核团的细胞构筑,本试验以8羽泰和乌鸡为研究对象,采用石蜡切片和HE染色技术,在光镜下观察分析乌鸡脑桥内耳蜗神经核、外展神经核及其副核、面神经核、三叉神经感觉主核、三叉神经运动核、蓝斑、蓝斑下核和脑桥中缝核的形态特征。结果表明:乌鸡脑桥的耳蜗神经核也分为耳蜗神经大细胞核、板核、角核3个亚核,核团内神经元以中型为主,耳蜗神经大细胞核相对比较发达,角核核周界不明显;外展神经核吻极与面神经核吻极几乎平行,外展神经副核与外展神经核非常接近,不易分开;面神经中间核不发达;三叉神经运动核没有明显的内、外、腹侧亚核之分;蓝斑细胞排列较密集,细胞多为圆形、椭圆形,以中型细胞为主;蓝斑下核细胞排列松散,核团界限不明显,绝大多数细胞为椭圆形、纺锤形,中型细胞占多数。中缝脑桥核位于脑桥后段中部以下的中缝区,神经元多为三角形、星形和椭圆形,突起很发达,多为巨型细胞。各核团的分布与北京鸭和鸡的大体相似。
In order to investigate the cytoarchitecture of the nuclei in central nervous system of silky fowl, the pons of ten silky fowl brainstems were studied by paraffin continual sections and haematoxylin-eosine (HE) staining. The morphologic characteristics of cochlear nuclei, abducens nuclei, abducens accessorius nucleus, facial nucleus, sensory principal nucleus of trigeminal nerve, motor nucleus of trigeminal nerve, locus ceruleus and nucleus subceruleus, and rapheal nucleus of pons were observed under the light microscope. The results of the present study showed that the cochlear nuclei were also divided into cochlear magnocellular nucleus, laminar nucleus and angular nucleus, but the neurons in this nuclear group were largely medium-sized. The cochlear magnocellular nuclei were relatively developed. The circumscription of the angular nucleus were not clear. The anterior poles of the abducens nuclei and the facial nucleus were mostly parallel. The abducens accessorius nucleus were remarkably close to the abducens nuclei. Facial intermedia nuclei were not developed fully. The motor nucleus of trigeminal nerve were not divided into three subnuclei. The neurons in locus ceruleus are relatively denseness. Most neurons of the locus ceruleus are medium-sized, round, oval. The nucleus subceruleus locates at the inferior, its verge was not obvious, the cells of which distribute sparsely, the neurons are mainly ellipse and spindle, and mostly are medium-sized. The nucleus raphes of pons situates at the lower part of the raphes area in the posterior segment of pons. The neurons are mainly triangle, starlike and ellipse, and mostly are giant neurons. The processes of the neurons are well developed. The distribution of all the nuclei were similar to Pekin duck and fowl.