东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

NDV对雏鸵鸟肾脏超微结构影响的研究

ISSN号：1000-6850
期刊名称：中国农学通报
时间：0
页码：61-65
分类：Q955[生物学—动物学]
作者机构：[1]临沂大学生命科学学院,山东临沂276000, [2]华中农业大学动物科技学院,武汉430070, [3]临沂市动物疫病预防控制中心,山东临沂276000, [4]诸城市畜牧局,山东诸城262200
相关基金：基金项目：国家自然科学基金资助项目（39970547,30471249）
相关项目：IL-21与胚胎期至育雏期鸵鸟免疫器官发生、发育的关系

关键词：雏鸵鸟, 卵巢, 显微, 超微结构, ostrich chicks, ovary, microstructure, ultrastructure

中文摘要：

为了解雏鸵鸟卵巢组织结构的形态学特点，采用解剖学及组织化学和透射电镜技术对90d非洲雏鸵鸟卵巢组织结构进行研究。结果表明：非洲雏鸵鸟卵巢内主要由原始卵泡、初级卵泡和次级卵泡组成，未出现成熟卵泡，各级卵泡卵母细胞的胞核大多为圆形，内有异染色质颗粒，核孔较明显，卵母细胞的胞质内有卵黄颗粒，胞质内含粗面内质网、线粒体、中心粒等细胞器；次级卵泡有一些特殊的超微结构，皮质颗粒出现在次级卵泡的卵母细胞内，并且主要位于靠近核周的胞质内，次级卵泡的卵黄膜较明显，并且形成放射带。另外，雏鸵鸟卵巢内网、连接网和外网上皮细胞的嗜碱性依次增强。结果提示，雏鸵鸟卵巢内次级卵泡的颗粒细胞的胞质内细胞器比初级卵泡的更丰富，尤其是具管状嵴的线粒体数量较多。雏鸵鸟卵巢内有卵巢网存在。

英文摘要：

The aim of this study was to reveal the tissue structure characters of the ovary in ostrich chicks. Healthy 90- day-old ostrich chicks were used in this study. This study was based on the anatomy traits, microstructure and ultrastructure of the ovary in ostrich chicks under both histochemical and transmission electron microscopical methods. The results showed that the ovary of ostrich chicks contained primordial follicles, primary follicles and secondary follicles, but no mature follicles. The oocytes in every grade of follicle were large, with an obvious nuclear pore and a heterochromatin mitochondria,centriole,etc. Some unique ultrastructural characteristics were observed in the secondary follicle,such as the cortical granule,which was located in cytoplasm beside the nucleus appeared first in the oocyte. Zona radiata appeared, and formed an obvious vitelline membrane. Additionally, our results showed that there were intraovadan rete, connecting rete, and extraovarian fete in the ovaries of ostrich chicks. Basophilic staining was observed with increasing amount from intraovarian rete to connecting fete,to extraobarian rete. These results indicated that the cellular organs in the cytoplasm of the secondary follicle are much more plentiful than the primary ovarian follicle,especially for the amount of tubiform cristal mitochondrion. Besides, there were rete ovarii in the ovary of the ostrich chicks.

同期刊论文项目