[目的]本试验目的是研究腿部正常的雏鸵鸟和患腿病雏鸵鸟其胫骨内部结构的差异,[方法]以90日龄健康正常的和患有腿病的雏鸵鸟为试验动物,利用骨密度测量、骨脱钙石蜡切片(HE染色)及骨磨片、扫描电镜观察分析腿部正常的雏鸵鸟和患腿病雏鸵鸟其胫骨形态学差异。[结果]患有腿病的雏鸵鸟的骨密度值显著低于正常的雏鸵鸟。与正常雏鸵鸟胫骨的组织结构相比,患腿病雏鸵鸟胫骨骨小梁面积明显减少,骨小梁变细,且出现断裂消失的现象;经扫描电镜观察,与正常雏鸵鸟相比,患腿病雏鸵鸟胫骨中的骨小梁多处出现微骨折现象,在骨组织观察面上、布满了大大小小的吸收坑。[结论]患有腿病的雏鸵鸟虽然腿病各有不同,但基本上都会出现骨量变少和骨小梁微断裂现象,其病因可能为骨质疏松。
[Objective] This study aimed to investigate the differences in morphological structure of tibias between sick and normal ostrich chicks. [Method] Normal and sick ostrich chicks aged 90 days were used in this research for bone mineral density determination, bone demineralization paraffin section preparation (HE staining), bone abrasive disc preparation and scanning electron microscopy (SEM) observation, to analyze the differences in microstructure and ultrastructure of tibias between sick ostrich chicks and normal ones. [Result] Bone mineral density of ostrich chicks suffering from leg disease was much lower than that of the normal ones. Compared with normal tibias, less trabeculae were observed in sick tibias, which were attenuated and began to disappear. In addition, trabeculae micro-fracture could be observed under scanning electron microscope; resorption pits on bone slices formed by osteoclast could be observed under scanning electronic microscope. [Conclusion] These results indicate that the sick tibias all have lower bone density and trabeculae microfracture phenomenon in tibias with different diseases, which might be resulted from osteoporosis.