健康彻丽山谷鸭在现在的学习被使用。色氨酸的不同剂量在是 fasted 以后 intraperitoneally 被注射到他们 4 h (上午 8:00 上午 12:00 ) 。一个小时以后,他们深深地被使麻木并且酒。小脑被移开使连续石蜡成为纵的节。streptavidin-biotin-peroxidase 建筑群(SABC ) 方法被用来在小脑学习像血清素的神经原的分发。所有电影被使用一个帮助计算机的图象分析系统分析。像血清素的神经原在小脑的 Purkinje 房间层仅仅是局部性的。在 200 和 100 mg kg 的像血清素的神经原的光密度一般水准 ? 1 个组比 0 mg kg 的显著地高级 ? 1 个组(P【0.01 ) 。像血清素的神经原在 Purkinje 房间层和那过多的色氨酸是分布式的这些结果表演能在小脑影响血清素的内容。
Healthy Cherry Valley ducks were used in the present study. Different doses of tryptophan were injected intraperitoneally to them after being fasted 4 h (8:00 a.m.-12:00 a.m.). One hour later, they were deeply anaesthetized and perfused. The cerebellum was removed to make serial paraffin longitudinal sections. The streptavidin-biotin-peroxidase complex (SABC) method was used to study the distribution of serotonin-like neurons in the cerebellum. All films were analysed by using a computer-assisted image analysis system. Serotonin-like neurons are only localized in cerebellar Purkinje cell layer. The optical density averages of serotonin-like neurons in 200 and 100 mg kg^-1 group are significantly higher than that of 0 mg kg^-1 group (P〈0.01). These results show that serotonin-like neurons are distributed in Purkinje cell layer and that excessive tryptophan can affect the content of serotonin in cerebellum.