滇重楼为延龄草科重楼属多年生草本植物,具有抗癌、止血等功效,市场应用前景广阔,但其种子在实际生产中存在萌发周期长和萌发率低等缺点。为了缩短滇重楼种子萌发时间,提高其萌发率,在三七愈伤组织上对滇重楼种子进行暗培养和光照培养试验。结果表明,在三七愈伤组织上进行暗培养能明显缩短滇重楼种子的萌发时间,最快在第10天就开始萌发,萌发高峰期在第20~30天,平均萌发率为12.5%。
Trillium family of Paris polyphylla Smith var. yunnanensis is a perennial herb, with cancer,bleeding and other effects,and broad market prospect, but exists in the actual production of seed germination cycle long and germination rate low. To shorten the seed germination time and increase the germination rate, this experiment cultures the seed of Paris ployphylla Smith var. yunnanensis on callus from Panax notoginseng in light circumstance and dark circumstance. The experiment results indicate that the dark culture can significantly shorten the germination time of the seed in the callus from Panax notoginseng, the fastest in tenth days to start germination,germination peak between 20-30 days, the average germination rate was 12.5%.