中文摘要：

线粒体是氧化还原信号通路的重要节点，然而，对于线粒体氧化还原变化的原位检测十分困难。作者选择秀丽隐杆线虫为模式生物，使用线粒体定位的氧化还原敏感探针Grxl-roGFP2对线虫进行标记。同时，为了快速比较多个样品间的氧化还原变化，建立了基于COPAS生物自动分选系统的自动定量检测方案。结合氧化还原敏感探针标记的转基因线虫品系，该方法能够快速检测RNA干扰以及线虫生长发育不同时期引起的线粒体氧化还原变化。

英文摘要：

Mitochondria have emerged as an important node for redox signaling. However, it is difficult to monitor the actual changes in redox status in situ. The authors selected a mitochondria-targeted redox-sensitive probe, Grxl-roGFP2, for expression in the nematode Caenorhabditis elegans. Using this probe, they developed a rapid, automated and quantitative method for examining changes in redox status in multiple samples with the COPAS （complex parametric analyser and sorter） Biosort, a flow cytometer designed to accommodate large objects such as C. elegans. This assay is capable of rapid, in situ monitoring of the mitochondrial redox status of biosensor-transgenic nematodes during nematode development and can be used to analyze the redox changes caused by RNA interference.